A Possible Role of the Aleurone Expressed Gene HvMAN1 in the Hydrolysis of the Cell Wall Mannans of the Starchy Endosperm in Germinating Hordeum vulgare L. Seeds

Iglesias Fernandez, Raquel and Pastor Mora, Elena and Vicente Carbajosa, Jesus and Carbonero Zalduegui, Pilar (2020). A Possible Role of the Aleurone Expressed Gene HvMAN1 in the Hydrolysis of the Cell Wall Mannans of the Starchy Endosperm in Germinating Hordeum vulgare L. Seeds. "Frontiers in Plant Science", v. 10 ; p. 1706. ISSN 1664-462X. https://doi.org/10.3389/fpls.2019.01706.

Description

Title: A Possible Role of the Aleurone Expressed Gene HvMAN1 in the Hydrolysis of the Cell Wall Mannans of the Starchy Endosperm in Germinating Hordeum vulgare L. Seeds
Author/s:
  • Iglesias Fernandez, Raquel
  • Pastor Mora, Elena
  • Vicente Carbajosa, Jesus
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Frontiers in Plant Science
Date: 20 January 2020
ISSN: 1664-462X
Volume: 10
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The barley endo-b-mannanase (MAN) gene family (HvMAN1-6) has been identified and the expression of its members analyzed throughout different plant organs, and upon grain development and germination. The HvMAN1 gene has been found to be highly expressed in developing and germinating grains. The MAN (EC 3.2.1.78) enzymatic activity gets a maximum in grains at 48 h of germination (post-germination event). Immunolocalization of mannan polymers in grains has revealed the presence of these polysaccharides in the endosperm cell walls (CWs). By mRNA in situ hybridization assays, the HvMAN1 transcripts have been localized to the aleurone layer, but not to the dead starchy endosperm cells. These data suggest that MAN1 is synthesized in the aleurone layer during early grain imbibition and moves potentially through the apoplast to the endosperm where the hydrolysis of the mannan polymers takes place after germination sensu stricto. Hence, mannans in the starchy endosperm CWs, besides their structural function, could be used as reserve compounds upon barley post-germination.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO2017-82873-RUnspecifiedUnspecifiedEstrés del retículo endoplásmico en plantas: nuevos componentes y aplicaciones biotecnológicas
Madrid Regional GovernmentPEJ16/BIO/TL-1451UnspecifiedUnspecifiedUnspecified
Universidad Politécnica de MadridVJIDOCUPM19ECBUnspecifiedUnspecifiedUnspecified

More information

Item ID: 57774
DC Identifier: http://oa.upm.es/57774/
OAI Identifier: oai:oa.upm.es:57774
DOI: 10.3389/fpls.2019.01706
Official URL: https://www.frontiersin.org/article/10.3389/fpls.2019.01706
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 23 Jan 2020 11:55
Last Modified: 23 Jan 2020 11:55
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