Synthetic and structural studies on Pyrularia pubera thionin: a single-residue mutation enhances activity against Gram-negative bacteria

Vila Perello, Miguel; Sánchez Vallet, Andrea; García Olmedo, Francisco; Molina Fernández, Antonio y Andreu, David (2003). Synthetic and structural studies on Pyrularia pubera thionin: a single-residue mutation enhances activity against Gram-negative bacteria. "Febs Letters", v. 536 (n. 1-3); pp. 215-219. ISSN 0014-5793. https://doi.org/10.1016/S0014-5793(03)00053-X.

Descripción

Título: Synthetic and structural studies on Pyrularia pubera thionin: a single-residue mutation enhances activity against Gram-negative bacteria
Autor/es:
  • Vila Perello, Miguel
  • Sánchez Vallet, Andrea
  • García Olmedo, Francisco
  • Molina Fernández, Antonio
  • Andreu, David
Tipo de Documento: Artículo
Título de Revista/Publicación: Febs Letters
Fecha: Febrero 2003
Volumen: 536
Materias:
Palabras Clave Informales: Thionin; Antimicrobial peptide; Oxidative folding; Plant
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

The thionin from Pyrularia pubera (Pp-TH), a 47-residue peptide with four internal disulfide bonds, was efficiently produced by chemical synthesis. Its antimicrobial activity in vitro against several representative pathogens (EC50=0.3–3.0 μM) was identical to that of natural Pp-TH. This peptide has a unique Asp32 instead of the consensus Arg found in other thionins of the same family. In order to evaluate the effect of this mutation, the Arg32 analogue (Pp-TH(D32R)) was also synthesized and showed a significant increase in antibiotic activity against several Gram-negative bacteria, whereas it retained the same activity against other pathogens. The overall structure of Pp-TH(D32R) was maintained, though a slight decrease in the helical content of the peptide was observed.

Más información

ID de Registro: 5805
Identificador DC: http://oa.upm.es/5805/
Identificador OAI: oai:oa.upm.es:5805
Identificador DOI: 10.1016/S0014-5793(03)00053-X
URL Oficial: http://www.sciencedirect.com/science/article/pii/S001457930300053X
Depositado por: Memoria Investigacion
Depositado el: 26 Ene 2011 11:01
Ultima Modificación: 20 Abr 2016 14:31
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