Relative Effects on Virulence of Mutations in the sap, pel, and hrp Loci of Erwinia chrysanthemi

Lopez Solanilla, Emilia and Llama Palacios, María Aránzazu and Collmer, Alan and García Olmedo, Francisco and Rodriguez Palenzuela, Pablo (2001). Relative Effects on Virulence of Mutations in the sap, pel, and hrp Loci of Erwinia chrysanthemi. "Molecular Plant Microbe Interactions", v. 14 (n. 3); pp. 386-393. ISSN 0894-0282.

Description

Title: Relative Effects on Virulence of Mutations in the sap, pel, and hrp Loci of Erwinia chrysanthemi
Author/s:
  • Lopez Solanilla, Emilia
  • Llama Palacios, María Aránzazu
  • Collmer, Alan
  • García Olmedo, Francisco
  • Rodriguez Palenzuela, Pablo
Item Type: Article
Título de Revista/Publicación: Molecular Plant Microbe Interactions
Date: March 2001
ISSN: 0894-0282
Volume: 14
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

We constructed strains of Erwinia chrysanthemi EC16 with múltiple mutations involving three virulence systems in this bacterium, namelypel (coding for the major pectate lyases pelABCE), hrp (hypersensitive response and pathogenicity), and sap (sensitivity to antimicrobial peptides). The relative effects on virulence of those mutations have been analyzed on potato tubers and chicory leaves. In potato tubers, the sap mutation (BT105) had a greater effect in the reduction of the virulence than the peí (CUCPB5006) and hrp (CUCPB5039) mutations. This reduction was similar to that observed in the pel-hrp double mutant (CUCPB5037). The analysis of the strains affected in Pel-Sap (BT106), Hrp-Sap (BT107), and Pel- Hrp-Sap (BT108) suggested that the effects of these mutations are additive. In chicory leaves, the mutation in the sap locus appeared to have a greater effect than in potato tubers. The competitive Índices of strains BT105, UM1005 (Peí"), CUCPB5039, and CUCPB5037 have been estimated in vivo and in vitro. These results indícate that the mutation in the hrp locus can be complemented in vivo by coinfection, whereas the mutations in peí and sap cannot.

More information

Item ID: 5831
DC Identifier: http://oa.upm.es/5831/
OAI Identifier: oai:oa.upm.es:5831
Official URL: http://apsjournals.apsnet.org/loi/mpmi
Deposited by: Memoria Investigacion
Deposited on: 01 Feb 2011 08:46
Last Modified: 20 Apr 2016 14:32
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