Relative Effects on Virulence of Mutations in the sap, pel, and hrp Loci of Erwinia chrysanthemi

Lopez Solanilla, Emilia; Llama Palacios, María Aránzazu; Collmer, Alan; García Olmedo, Francisco y Rodriguez Palenzuela, Pablo (2001). Relative Effects on Virulence of Mutations in the sap, pel, and hrp Loci of Erwinia chrysanthemi. "Molecular Plant Microbe Interactions", v. 14 (n. 3); pp. 386-393. ISSN 0894-0282.

Descripción

Título: Relative Effects on Virulence of Mutations in the sap, pel, and hrp Loci of Erwinia chrysanthemi
Autor/es:
  • Lopez Solanilla, Emilia
  • Llama Palacios, María Aránzazu
  • Collmer, Alan
  • García Olmedo, Francisco
  • Rodriguez Palenzuela, Pablo
Tipo de Documento: Artículo
Título de Revista/Publicación: Molecular Plant Microbe Interactions
Fecha: Marzo 2001
Volumen: 14
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

We constructed strains of Erwinia chrysanthemi EC16 with múltiple mutations involving three virulence systems in this bacterium, namelypel (coding for the major pectate lyases pelABCE), hrp (hypersensitive response and pathogenicity), and sap (sensitivity to antimicrobial peptides). The relative effects on virulence of those mutations have been analyzed on potato tubers and chicory leaves. In potato tubers, the sap mutation (BT105) had a greater effect in the reduction of the virulence than the peí (CUCPB5006) and hrp (CUCPB5039) mutations. This reduction was similar to that observed in the pel-hrp double mutant (CUCPB5037). The analysis of the strains affected in Pel-Sap (BT106), Hrp-Sap (BT107), and Pel- Hrp-Sap (BT108) suggested that the effects of these mutations are additive. In chicory leaves, the mutation in the sap locus appeared to have a greater effect than in potato tubers. The competitive Índices of strains BT105, UM1005 (Peí"), CUCPB5039, and CUCPB5037 have been estimated in vivo and in vitro. These results indícate that the mutation in the hrp locus can be complemented in vivo by coinfection, whereas the mutations in peí and sap cannot.

Más información

ID de Registro: 5831
Identificador DC: http://oa.upm.es/5831/
Identificador OAI: oai:oa.upm.es:5831
URL Oficial: http://apsjournals.apsnet.org/loi/mpmi
Depositado por: Memoria Investigacion
Depositado el: 01 Feb 2011 08:46
Ultima Modificación: 20 Abr 2016 14:32
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