Snakin-1, a Peptide from Potato That Is Active Against Plant Pathogens

Segura, Ana; Moreno, Manuel; Madueño, Francisco; Molina Fernández, Antonio y García Olmedo, Francisco (1999). Snakin-1, a Peptide from Potato That Is Active Against Plant Pathogens. "Molecular Plant-Microbe Interactions", v. 12 (n. 1); pp. 16-23. ISSN 0894-0282.

Descripción

Título: Snakin-1, a Peptide from Potato That Is Active Against Plant Pathogens
Autor/es:
  • Segura, Ana
  • Moreno, Manuel
  • Madueño, Francisco
  • Molina Fernández, Antonio
  • García Olmedo, Francisco
Tipo de Documento: Artículo
Título de Revista/Publicación: Molecular Plant-Microbe Interactions
Fecha: Febrero 1999
Volumen: 12
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

A new type of antimicrobial peptide, snakin-1 (SN1), has been isolated from potato tubers and found to be active, at concentrations <10 uM, against bacterial and fungal pathogens from potato and other plant species. The action of SN1 and potato defensin PTH1 was synergistic against the bacterium Clavibacter michiganensis subsp. sepedonicus and additive against the fungus Botrytis cinérea. Snakin-1 causes aggregation of both gram-positive and gram-negative bacteria. The peptide has 63 amino acid residues (Mr 6,922), 12 of which are cysteines, and is unrelated to any previously isolated protein, although it is homologous to amino acid sequences deduced from cloned cDNAs that encode gibberellin-inducible mRNAs and has some sequence motifs in common with kistrin and other hemotoxic snake venoms. A degenerate oligonucleotide probé based on the internal sequence CCEECKC has been used to clone an SN1 cDNA. With the cDNA used as probé, one copy of the StSNl gene per haploid genome has been estimated and expression of the gene has been detected in tubers, stems, axillary buds, and young floral buds. Expression levéis in petáis and carpels from fully developed flowers were much higher than in sepáis and stamens. The expression pattern of gene StSNl suggests that protein SN1 may be a component of constitutive defense barriers, especially those of storage and reproductive plant organs.

Más información

ID de Registro: 5834
Identificador DC: http://oa.upm.es/5834/
Identificador OAI: oai:oa.upm.es:5834
URL Oficial: http://apsjournals.apsnet.org/loi/mpmi
Depositado por: Memoria Investigacion
Depositado el: 01 Feb 2011 10:20
Ultima Modificación: 20 Abr 2016 14:32
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