Snakin-1, a Peptide from Potato That Is Active Against Plant Pathogens

Segura, Ana and Moreno, Manuel and Madueño, Francisco and Molina Fernández, Antonio and García Olmedo, Francisco (1999). Snakin-1, a Peptide from Potato That Is Active Against Plant Pathogens. "Molecular Plant-Microbe Interactions", v. 12 (n. 1); pp. 16-23. ISSN 0894-0282.

Description

Title: Snakin-1, a Peptide from Potato That Is Active Against Plant Pathogens
Author/s:
  • Segura, Ana
  • Moreno, Manuel
  • Madueño, Francisco
  • Molina Fernández, Antonio
  • García Olmedo, Francisco
Item Type: Article
Título de Revista/Publicación: Molecular Plant-Microbe Interactions
Date: February 1999
ISSN: 0894-0282
Volume: 12
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

A new type of antimicrobial peptide, snakin-1 (SN1), has been isolated from potato tubers and found to be active, at concentrations <10 uM, against bacterial and fungal pathogens from potato and other plant species. The action of SN1 and potato defensin PTH1 was synergistic against the bacterium Clavibacter michiganensis subsp. sepedonicus and additive against the fungus Botrytis cinérea. Snakin-1 causes aggregation of both gram-positive and gram-negative bacteria. The peptide has 63 amino acid residues (Mr 6,922), 12 of which are cysteines, and is unrelated to any previously isolated protein, although it is homologous to amino acid sequences deduced from cloned cDNAs that encode gibberellin-inducible mRNAs and has some sequence motifs in common with kistrin and other hemotoxic snake venoms. A degenerate oligonucleotide probé based on the internal sequence CCEECKC has been used to clone an SN1 cDNA. With the cDNA used as probé, one copy of the StSNl gene per haploid genome has been estimated and expression of the gene has been detected in tubers, stems, axillary buds, and young floral buds. Expression levéis in petáis and carpels from fully developed flowers were much higher than in sepáis and stamens. The expression pattern of gene StSNl suggests that protein SN1 may be a component of constitutive defense barriers, especially those of storage and reproductive plant organs.

More information

Item ID: 5834
DC Identifier: http://oa.upm.es/5834/
OAI Identifier: oai:oa.upm.es:5834
Official URL: http://apsjournals.apsnet.org/loi/mpmi
Deposited by: Memoria Investigacion
Deposited on: 01 Feb 2011 10:20
Last Modified: 20 Apr 2016 14:32
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