Dithiothreitol increases f3-glucuronidase accumulation in transformed tobacco (Nicotiana tabacum) protoplasts without altering their viability or the synthesis and export of cellular proteins

Piñeiro Galvin, Manuel and Alamillo, Josefa M. and García Olmedo, Francisco (1999). Dithiothreitol increases f3-glucuronidase accumulation in transformed tobacco (Nicotiana tabacum) protoplasts without altering their viability or the synthesis and export of cellular proteins. "Plant Cell Report", v. 18 (n. 6); pp. 505-508. ISSN 0721-7714. https://doi.org/10.1007/s002990050612.

Description

Title: Dithiothreitol increases f3-glucuronidase accumulation in transformed tobacco (Nicotiana tabacum) protoplasts without altering their viability or the synthesis and export of cellular proteins
Author/s:
  • Piñeiro Galvin, Manuel
  • Alamillo, Josefa M.
  • García Olmedo, Francisco
Item Type: Article
Título de Revista/Publicación: Plant Cell Report
Date: February 1999
ISSN: 0721-7714
Volume: 18
Subjects:
Freetext Keywords: Dithiothreitol - β-Glucuronidase - Redox modulation - Tobacco protoplasts
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The effect of dithiothreitol (DTT) on the expression of the β-glucuronidase (GUS) reporter gene under the control of the CaMV-35 S promoter has been investigated by radioactive labelling and immunoprecipitation of the enzyme in protoplasts from stably transformed tobacco plants and compared with that observed in protoplasts transiently expressing the same gene construct. An increase in net accumulation of GUS during the culture period in response to externally added DTT (2 mm) was observed both in protoplasts from transformed tobacco plants and in electroporated protoplasts. DTT had no effect on rate of degradation of the mature GUS protein, as shown in a pulse-chase experiment. Relevant aspects of protoplast physiology, such as viability, synthesis of 35S-labelled cellular proteins, or synthesis and export of pathogenesis-related proteins (one putative chitinase and two 1,3-β-glucanases) were not affected by the reducing reagent

More information

Item ID: 5835
DC Identifier: http://oa.upm.es/5835/
OAI Identifier: oai:oa.upm.es:5835
DOI: 10.1007/s002990050612
Official URL: http://www.springerlink.com/content/4krrvhnq6cf95f6l/
Deposited by: Memoria Investigacion
Deposited on: 01 Feb 2011 11:06
Last Modified: 20 Apr 2016 14:32
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