Dithiothreitol increases f3-glucuronidase accumulation in transformed tobacco (Nicotiana tabacum) protoplasts without altering their viability or the synthesis and export of cellular proteins

Piñeiro Galvin, Manuel; Alamillo, Josefa M. y García Olmedo, Francisco (1999). Dithiothreitol increases f3-glucuronidase accumulation in transformed tobacco (Nicotiana tabacum) protoplasts without altering their viability or the synthesis and export of cellular proteins. "Plant Cell Report", v. 18 (n. 6); pp. 505-508. ISSN 0721-7714. https://doi.org/10.1007/s002990050612.

Descripción

Título: Dithiothreitol increases f3-glucuronidase accumulation in transformed tobacco (Nicotiana tabacum) protoplasts without altering their viability or the synthesis and export of cellular proteins
Autor/es:
  • Piñeiro Galvin, Manuel
  • Alamillo, Josefa M.
  • García Olmedo, Francisco
Tipo de Documento: Artículo
Título de Revista/Publicación: Plant Cell Report
Fecha: Febrero 1999
Volumen: 18
Materias:
Palabras Clave Informales: Dithiothreitol - β-Glucuronidase - Redox modulation - Tobacco protoplasts
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

The effect of dithiothreitol (DTT) on the expression of the β-glucuronidase (GUS) reporter gene under the control of the CaMV-35 S promoter has been investigated by radioactive labelling and immunoprecipitation of the enzyme in protoplasts from stably transformed tobacco plants and compared with that observed in protoplasts transiently expressing the same gene construct. An increase in net accumulation of GUS during the culture period in response to externally added DTT (2 mm) was observed both in protoplasts from transformed tobacco plants and in electroporated protoplasts. DTT had no effect on rate of degradation of the mature GUS protein, as shown in a pulse-chase experiment. Relevant aspects of protoplast physiology, such as viability, synthesis of 35S-labelled cellular proteins, or synthesis and export of pathogenesis-related proteins (one putative chitinase and two 1,3-β-glucanases) were not affected by the reducing reagent

Más información

ID de Registro: 5835
Identificador DC: http://oa.upm.es/5835/
Identificador OAI: oai:oa.upm.es:5835
Identificador DOI: 10.1007/s002990050612
URL Oficial: http://www.springerlink.com/content/4krrvhnq6cf95f6l/
Depositado por: Memoria Investigacion
Depositado el: 01 Feb 2011 11:06
Ultima Modificación: 20 Abr 2016 14:32
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