Selective disulphide linkage of plant thionins with other proteins

Piñeiro Galvin, Manuel and Diaz Rodriguez, Isabel and Rodriguez Palenzuela, Pablo and Titarenko, Elena and García Olmedo, Francisco (1995). Selective disulphide linkage of plant thionins with other proteins. "Febs Letters", v. 369 (n. 2-3); pp. 239-242. ISSN 0014-5793. https://doi.org/10.1016/0014-5793(95)00759-3.

Description

Title: Selective disulphide linkage of plant thionins with other proteins
Author/s:
  • Piñeiro Galvin, Manuel
  • Diaz Rodriguez, Isabel
  • Rodriguez Palenzuela, Pablo
  • Titarenko, Elena
  • García Olmedo, Francisco
Item Type: Article
Título de Revista/Publicación: Febs Letters
Date: August 1995
ISSN: 0014-5793
Volume: 369
Subjects:
Freetext Keywords: Thionin; Toxin; Neomycin phosphotransferase; β-Glucuronidase; Disulphide bridge; Pseudomonas solanacearum
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Thionins are shown to form disulphide linkages with other proteins. The reaction with bacterial enzymes β-glucuronidase and neomycin phosphotransferase II could be prevented and reversed with dithiothreitol and blocked with N-ethylmaleimide. Other cysteine-rich low-molecular-weight toxic peptides from plants (LTP-3 from barley and P19 from potato) did not react as the thionins. Certain systeine-containing proteins, such bovine serum albumin, ovalbumin and cytochrome c, reacted with thionins, while others, including carbonic anhydrase, soybean trypsin inhibitor, bovine-lung trypsin inhibitor and phosphorylase B did not. Selectivity of the reaction with a periplasmic component of the phytopathogenic bacterium Pseudomonas solanacearum was also shown.

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