A fatty-acid-binding protein from wheat kernels

Castagnaro, Atilio and García Olmedo, Francisco (1994). A fatty-acid-binding protein from wheat kernels. "Febs Letters", v. 349 (n. 1); pp. 117-119. ISSN 0014-5793. https://doi.org/10.1016/0014-5793(94)00660-1.

Description

Title: A fatty-acid-binding protein from wheat kernels
Author/s:
  • Castagnaro, Atilio
  • García Olmedo, Francisco
Item Type: Article
Título de Revista/Publicación: Febs Letters
Date: July 1994
ISSN: 0014-5793
Volume: 349
Subjects:
Freetext Keywords: Cereal; Fatty acid binding protein; Plant, Wheat
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

A protein of about 7 kDa (W-FABP) has been isolated from mature wheat kernels by H2O extraction and gel filtration of the extract, followed by two steps of high-performance liquid chromatography. The N-terminal amino acid sequence has been determined up to the 28th residue and found to be identical (except for positions 4 and 5) to that deduced from a barley cDNA (EMBL X15257), which had been improperly classified as a non-specific lipid transfer protein (LTP2). Similarly with LTPs, W-FABP does bind fatty acids, but in contrast, it is not significantly homologous to LTPs, it is not recognized by LTP antibodies, it has a more acidic isoelectric point (pH < 6.8 vs. pH> 9.6), and it does not show antibiotic properties.

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