Pseudothionin-St1. A potato peptide active against potato pathogens.

Moreno, Manuel and Segura, Ana and García Olmedo, Francisco (1994). Pseudothionin-St1. A potato peptide active against potato pathogens.. "The FEBS Journal. European Journal of Biochemistry", v. 223 (n. 1); pp. 135-139. ISSN 1742-4658. https://doi.org/10.1111/j.1432-1033.1994.tb18974.x.

Description

Title: Pseudothionin-St1. A potato peptide active against potato pathogens.
Author/s:
  • Moreno, Manuel
  • Segura, Ana
  • García Olmedo, Francisco
Item Type: Article
Título de Revista/Publicación: The FEBS Journal. European Journal of Biochemistry
Date: July 1994
ISSN: 1742-4658
Volume: 223
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

A 5-kDa polypeptide, pseudothionin Solanum tuberosum 1(Pth-St1), which was active against Clavibacter michiganensis subspecies sepedonicus, a bacterial pathogen of potatoes, has been purified from the buffer-insoluble fraction of potato tubers by salt extraction and HPCL. Pth-St1 was also active against other potato pathogens tested (Pseudomonas solanacearum and Fusarium solani). The N-terminal amino acid sequence of this peptide was identical (except for a N/H substitution at position 2) to that deduced from a previously reported cDNA sequence (EMBL accession number X-13180), which had been misclassified as a Bowman-Birk protease inhibitor. Pth-St1 did not inhibit either trypsin or insect α-amylase activities, and, in contrast with true thionins, did not affect cell-free protein synthesis or β-glucuronidase activity. Northern-blot and tissue-print analyses showed that steady-state mRNA levels were highest in flowers (especially in petals), followed by tubers (especially in the epidermal cell layers and in leaf primordia), stems and leaves. Infection of leaves with a bacterial pathogen suspended in 10 mM MgCl2 switched off the gene, whereas mock inoculation with 10 mM MgCl2 alone induced higher mRNA levels.

More information

Item ID: 5862
DC Identifier: http://oa.upm.es/5862/
OAI Identifier: oai:oa.upm.es:5862
DOI: 10.1111/j.1432-1033.1994.tb18974.x
Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1994.tb18974.x/full
Deposited by: Memoria Investigacion
Deposited on: 02 Feb 2011 10:45
Last Modified: 20 Apr 2016 14:34
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