cDNA cloning and nucleotide sequences of a1 and a2 thionins from hexaploid wheat endosperm

Castagnaro, Atilio and Maraña, Carmen and Carbonero Zalduegui, Pilar and García Olmedo, Francisco (1994). cDNA cloning and nucleotide sequences of a1 and a2 thionins from hexaploid wheat endosperm. "Plant Physiology", v. 106 (n. 3); pp. 1221-1222. ISSN 0032-0889. https://doi.org/10.1104/pp.106.3.1221.

Description

Title: cDNA cloning and nucleotide sequences of a1 and a2 thionins from hexaploid wheat endosperm
Author/s:
  • Castagnaro, Atilio
  • Maraña, Carmen
  • Carbonero Zalduegui, Pilar
  • García Olmedo, Francisco
Item Type: Article
Título de Revista/Publicación: Plant Physiology
Date: November 1994
ISSN: 0032-0889
Volume: 106
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Thionins are homologous ‘ys-nch proteins Of about kD that have been isolated from different tissues in a wide range Table 1. Characteristics ofcDNAs encoding a, and az thionins from hexaplo;d ,,,heat of plant taxa and are active against plant pathogens both in vitro and in vivo (Fernindez de Caleya et al., 1972; Garcia- Olmedo et al., 1992; Carmona et al., 1993). The available amino acid sequences (either directly determined or deduced from cDNAs) have been classified into five well-defined Organism: Loci Products: Relevant Feature of the Products: Triticum aestivum L. cv Chinese Spring. Pur-57, a, thionin; Pur-DI, a2 thionin. structural types. Two of these types, I and V, are present in wheat endosperm (Castagnaro et al., 1992; Garcia-Olmedo et al., 1992). Type I corresponds to those thionins included in the original purothionin mixture obtained from wheat flour by Balls and co-workers (Balls et al., 1942). This mixture was resolved through CM-cellulose chromatography into two components, a and /3 purothionins (Redman and Fisher, 1968). The presence of structural genes for the a purothionin fraction in the long arms of chromosomes 1B and 1D of hexaploid wheat and for the /3 component in the long arm of chromosome 1A was subsequently demonstrated (Fernindez de Caleya et al., 1976). The a purothionin fraction was later resolved by ion-exchange chromatography into two compo- nents, a1 and az, whose amino acid sequences differ in six positions (Jones and Mak, 1977) and whose genes are respec- tively located on chromosomes 1B and 1D (Fernandez de Caleya et al., 1976).

More information

Item ID: 5863
DC Identifier: http://oa.upm.es/5863/
OAI Identifier: oai:oa.upm.es:5863
DOI: 10.1104/pp.106.3.1221
Official URL: http://www.plantphysiol.org/content/vol106/issue3/#DEVELOPMENT_AND_GROWTH_REGULATION
Deposited by: Memoria Investigacion
Deposited on: 02 Feb 2011 11:03
Last Modified: 20 Apr 2016 14:34
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