Lipid transfer proteins (nsLTPs) from barley and maize leaves are potent inhibitors of bacterial and fungal plant pathogens

Molina Fernández, Antonio; Segura, Ana y García Olmedo, Francisco (1993). Lipid transfer proteins (nsLTPs) from barley and maize leaves are potent inhibitors of bacterial and fungal plant pathogens. "Febs Letters", v. 136 (n. 2); pp. 119-122. ISSN 0014-5793. https://doi.org/10.1016/0014-5793(93)81198-9.

Descripción

Título: Lipid transfer proteins (nsLTPs) from barley and maize leaves are potent inhibitors of bacterial and fungal plant pathogens
Autor/es:
  • Molina Fernández, Antonio
  • Segura, Ana
  • García Olmedo, Francisco
Tipo de Documento: Artículo
Título de Revista/Publicación: Febs Letters
Fecha: Enero 1993
Volumen: 136
Materias:
Palabras Clave Informales: Lipid transfer protein; Plant pathogen; Thionin
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Four homogeneous proteins (Cw18, Cw20, Cw21, Cw22) were isolated from etiolated barley leaves by extraction of the insoluble pellet from a Tris-HCl (pH 7.5) homogenate with 1.5 M LiCl and fractionation by reverse-phase high-performance liquid chromatography. All 4 proteins inhibited growth of the pathogen Clavibacter michiganensis subsp. sepedonicus (EC50S = 1−3 × 10−7 M) and had closely related N-terminal amino acid sequences. The complete amino acid sequences of proteins Cw18 and Cw21 were determined and found to be homologous to previously described, non-specific lipid transfer proteins from plants (32–62% identical positions). The proteins also inhibited growth of the bacterial pathogen Pseudomonas solanacearum (EC50s = 3–6 × 10−7 M) and the fungus Furium solani (EC50s = 3–20 × 10−6 M). A homologous protein from maize leaves (Cw41) was purified in a similar manner and also found to have inhibitory properties, A synergistic effect against the fungus was observed when protein Cw21, was combined with thionins. A defense role for non-specific lipid transfer proteins from plants is proposed.

Más información

ID de Registro: 5869
Identificador DC: http://oa.upm.es/5869/
Identificador OAI: oai:oa.upm.es:5869
Identificador DOI: 10.1016/0014-5793(93)81198-9
URL Oficial: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44F7MCW-6P&_user=885385&_coverDate=01/25/1993&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_searchStrId=1627928392&_rerunOrigin=google&_acct=C000047350&_version=1&_
Depositado por: Memoria Investigacion
Depositado el: 02 Feb 2011 12:33
Ultima Modificación: 20 Abr 2016 14:34
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