Effects of thionins on β-glucuronidase in vitro and in plant protoplasts

Diaz Rodriguez, Isabel and Carmona Quiles, Maria Jose and García Olmedo, Francisco (1992). Effects of thionins on β-glucuronidase in vitro and in plant protoplasts. "Febs Letters", v. 296 (n. 3); pp. 279-282. ISSN 0014-5793. https://doi.org/10.1016/0014-5793(92)80304-Y.

Description

Title: Effects of thionins on β-glucuronidase in vitro and in plant protoplasts
Author/s:
  • Diaz Rodriguez, Isabel
  • Carmona Quiles, Maria Jose
  • García Olmedo, Francisco
Item Type: Article
Título de Revista/Publicación: Febs Letters
Date: January 1992
ISSN: 0014-5793
Volume: 296
Subjects:
Freetext Keywords: β-Glucuronidase; Thionin; Tobacco protoplast; Transient expression
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (170kB) | Preview

Abstract

Thionins cause the irreversible inactivation of β-glucuronidase (GUS) in vitro in a dose- and time-dependent manner. The enzyme is also sensitive to externally added thionins when expressed in the cytoplasmic compartment of tobacco protoplasts transformed with the Gus gene under the 35S promoter of the cauliflower mosaic virus. In protoplasts transformed with the Gus gene fused to a signal peptide, where GUS is translocated into the lumen of the endoplasmic reticulum, the activity is significantly increased both by externally-added and by transiently-expressed thionin, suggesting that it interferes with GUS secretion

  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM