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A dimeric inhibitor of insect a-amylase from barley. Cloning of the cDNA and identification of the protein
Citation
Lazaro, Ana and Sánchez-Monge Laguna de Rins, Rosa and Salcedo Duran, Gabriel and Paz Ares, Javier and Carbonero Zalduegui, Pilar (1988). A dimeric inhibitor of insect a-amylase from barley. Cloning of the cDNA and identification of the protein. "The FEBS Journal. European Journal of Biochemistry", v. 172 (n. 1); pp. 129-134. ISSN 0014-2956. https://doi.org/10.1111/j.1432-1033.1988.tb13864.x.
Description
| Title: | A dimeric inhibitor of insect a-amylase from barley. Cloning of the cDNA and identification of the protein |
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| Author/s: |
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| Item Type: | Article |
| Título de Revista/Publicación: | The FEBS Journal. European Journal of Biochemistry |
| Date: | February 1988 |
| ISSN: | 0014-2956 |
| Volume: | 172 |
| Subjects: | |
| Faculty: | E.T.S.I. Agrónomos (UPM) [antigua denominación] |
| Department: | Biotecnologia [hasta 2014] |
| Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
Abstract
A cDNA clone, designated pUP-44, whose longest open reading frame codes for a protein that is homologous to the wheat α-amylase inhibitors, has been isolated from a library obtained from developing barley endosperm. The deduced sequence for the mature protein, which is 122 residues long, is preceded by a sequence of 30 residues which has the typical features of a signal peptide. A closely corresponding protein, designated BDAI-1, has been isolated from mature endosperm. BDAI-1 behaves as a dimer and inhibits the α-amylase from the insect Tenebrio molitor at concentrations that have no effect on salivary or pancreatic α-amylases.
More information
| Item ID: | 5883 |
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| DC Identifier: | http://oa.upm.es/5883/ |
| OAI Identifier: | oai:oa.upm.es:5883 |
| DOI: | 10.1111/j.1432-1033.1988.tb13864.x |
| Official URL: | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1988.tb13864.x/abstract |
| Deposited by: | Memoria Investigacion |
| Deposited on: | 03 Feb 2011 10:40 |
| Last Modified: | 20 Apr 2016 14:34 |
