A dimeric inhibitor of insect a-amylase from barley. Cloning of the cDNA and identification of the protein

Lazaro, Ana and Sánchez-Monge Laguna de Rins, Rosa and Salcedo Duran, Gabriel and Paz Ares, Javier and Carbonero Zalduegui, Pilar (1988). A dimeric inhibitor of insect a-amylase from barley. Cloning of the cDNA and identification of the protein. "The FEBS Journal. European Journal of Biochemistry", v. 172 (n. 1); pp. 129-134. ISSN 0014-2956. https://doi.org/10.1111/j.1432-1033.1988.tb13864.x.

Description

Title: A dimeric inhibitor of insect a-amylase from barley. Cloning of the cDNA and identification of the protein
Author/s:
  • Lazaro, Ana
  • Sánchez-Monge Laguna de Rins, Rosa
  • Salcedo Duran, Gabriel
  • Paz Ares, Javier
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: The FEBS Journal. European Journal of Biochemistry
Date: February 1988
ISSN: 0014-2956
Volume: 172
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

A cDNA clone, designated pUP-44, whose longest open reading frame codes for a protein that is homologous to the wheat α-amylase inhibitors, has been isolated from a library obtained from developing barley endosperm. The deduced sequence for the mature protein, which is 122 residues long, is preceded by a sequence of 30 residues which has the typical features of a signal peptide. A closely corresponding protein, designated BDAI-1, has been isolated from mature endosperm. BDAI-1 behaves as a dimer and inhibits the α-amylase from the insect Tenebrio molitor at concentrations that have no effect on salivary or pancreatic α-amylases.

More information

Item ID: 5883
DC Identifier: http://oa.upm.es/5883/
OAI Identifier: oai:oa.upm.es:5883
DOI: 10.1111/j.1432-1033.1988.tb13864.x
Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1988.tb13864.x/abstract
Deposited by: Memoria Investigacion
Deposited on: 03 Feb 2011 10:40
Last Modified: 20 Apr 2016 14:34
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