Characterization of cDNA clones of the family of trypsin/α-amylase inhibitors (CM-proteins) in barley (Hordeum vulgare L.)

Paz Ares, Javier; Ponz Ascaso, Fernando; Rodriguez Palenzuela, Pablo; Lazaro, Ana; Hernandez Lucas, Carlos; García Olmedo, Francisco y Carbonero Zalduegui, Pilar (1986). Characterization of cDNA clones of the family of trypsin/α-amylase inhibitors (CM-proteins) in barley (Hordeum vulgare L.). "TAG Theorical and Applied Genetics", v. 71 (n. 6); pp. 842-846. ISSN 0040-5752. https://doi.org/10.1007/BF00276427.

Descripción

Título: Characterization of cDNA clones of the family of trypsin/α-amylase inhibitors (CM-proteins) in barley (Hordeum vulgare L.)
Autor/es:
  • Paz Ares, Javier
  • Ponz Ascaso, Fernando
  • Rodriguez Palenzuela, Pablo
  • Lazaro, Ana
  • Hernandez Lucas, Carlos
  • García Olmedo, Francisco
  • Carbonero Zalduegui, Pilar
Tipo de Documento: Artículo
Título de Revista/Publicación: TAG Theorical and Applied Genetics
Fecha: 1986
Volumen: 71
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Recombinants encoding members of the trypsin/-amylase inhibitors family (also designated CM-proteins) were selected from a cDNA library prepared from developing barley endosperm. Inserts in two of the clones, pUP-13 and pUP-38, were sequenced and found to encode proteins which clearly belong to this family, as judged from the extensive homology of the deduced sequences with that of the barley trypsin inhibitor CMe, the only member of the group for which a complete amino acid sequence has been obtained by direct protein sequencing. These results, together with previously obtained N-terminal sequences of purified CM-proteins, imply that there are at least six different members of this dispersed gene family in barley. The relationship of this protein family to the B-3 hordein and to reserve prolamins from related species is discussed in terms of their genome structure and evolution.

Más información

ID de Registro: 5900
Identificador DC: http://oa.upm.es/5900/
Identificador OAI: oai:oa.upm.es:5900
Identificador DOI: 10.1007/BF00276427
URL Oficial: http://www.springerlink.com/content/v00481glx9118222/
Depositado por: Memoria Investigacion
Depositado el: 07 Feb 2011 08:56
Ultima Modificación: 20 Abr 2016 14:35
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