Characterization of cDNA clones of the family of trypsin/α-amylase inhibitors (CM-proteins) in barley (Hordeum vulgare L.)

Paz Ares, Javier and Ponz Ascaso, Fernando and Rodriguez Palenzuela, Pablo and Lazaro, Ana and Hernandez Lucas, Carlos and García Olmedo, Francisco and Carbonero Zalduegui, Pilar (1986). Characterization of cDNA clones of the family of trypsin/α-amylase inhibitors (CM-proteins) in barley (Hordeum vulgare L.). "TAG Theorical and Applied Genetics", v. 71 (n. 6); pp. 842-846. ISSN 0040-5752. https://doi.org/10.1007/BF00276427.

Description

Title: Characterization of cDNA clones of the family of trypsin/α-amylase inhibitors (CM-proteins) in barley (Hordeum vulgare L.)
Author/s:
  • Paz Ares, Javier
  • Ponz Ascaso, Fernando
  • Rodriguez Palenzuela, Pablo
  • Lazaro, Ana
  • Hernandez Lucas, Carlos
  • García Olmedo, Francisco
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: TAG Theorical and Applied Genetics
Date: 1986
ISSN: 0040-5752
Volume: 71
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Recombinants encoding members of the trypsin/-amylase inhibitors family (also designated CM-proteins) were selected from a cDNA library prepared from developing barley endosperm. Inserts in two of the clones, pUP-13 and pUP-38, were sequenced and found to encode proteins which clearly belong to this family, as judged from the extensive homology of the deduced sequences with that of the barley trypsin inhibitor CMe, the only member of the group for which a complete amino acid sequence has been obtained by direct protein sequencing. These results, together with previously obtained N-terminal sequences of purified CM-proteins, imply that there are at least six different members of this dispersed gene family in barley. The relationship of this protein family to the B-3 hordein and to reserve prolamins from related species is discussed in terms of their genome structure and evolution.

More information

Item ID: 5900
DC Identifier: http://oa.upm.es/5900/
OAI Identifier: oai:oa.upm.es:5900
DOI: 10.1007/BF00276427
Official URL: http://www.springerlink.com/content/v00481glx9118222/
Deposited by: Memoria Investigacion
Deposited on: 07 Feb 2011 08:56
Last Modified: 20 Apr 2016 14:35
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