Lipid binding proteins from the endosperms of wheat and Oats

Ponz Ascaso, Fernando and Hernandez Lucas, Carlos and Carbonero Zalduegui, Pilar and García Olmedo, Francisco (1984). Lipid binding proteins from the endosperms of wheat and Oats. "Phytochemistry", v. 23 (n. 10); pp. 2179-2181. ISSN 0031-9422. https://doi.org/10.1016/S0031-9422(00)80515-4.

Description

Title: Lipid binding proteins from the endosperms of wheat and Oats
Author/s:
  • Ponz Ascaso, Fernando
  • Hernandez Lucas, Carlos
  • Carbonero Zalduegui, Pilar
  • García Olmedo, Francisco
Item Type: Article
Título de Revista/Publicación: Phytochemistry
Date: 1984
ISSN: 0031-9422
Volume: 23
Subjects:
Freetext Keywords: Triticum; Avena; Gramineae; wheat; oats; lipid binding protein; endosperm
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

A protein, designated lipid binding protein (LBP), has been purified from the petrol extracts of wheat and oat endosperms by hydrochloric acid precipitation in a non-polar medium and preparative electrophoresis. The purified LBP appeared to be homogeneous both by electrophoresis in sodium dodecyl sulphate-polyacrylamide (SDS-PAGE) gels (MW ca 14 500) and by electrophoresis (PAGE) at pH 3.2. The amino acid composition indicates a high degree of homology between the LBPs from the two sources, as judged by the indexes of Cornish-Bowden and of Harris and Teller. As in the case of thionin, a previously characterized polypeptide from the ether extract, LBP becomes ether-insoluble, chloroform-soluble by precipitation with acetone, and solubility in ether is restored by binding of digalactosyl diglyceride to the chloroform-soluble form.

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