The CM-proteins from cereal endosperm: Immunochemical relationships

Paz Ares, Javier; Hernandez Lucas, Carlos; Salcedo Duran, Gabriel; Aragoncillo Ballesteros, Cipriano; Ponz Ascaso, Fernando y García Olmedo, Francisco (1983). The CM-proteins from cereal endosperm: Immunochemical relationships. "Journal of Experimental Botany", v. 34 (n. 4); pp. 388-395. ISSN 0022-0957. https://doi.org/10.1093/jxb/34.4.388.

Descripción

Título: The CM-proteins from cereal endosperm: Immunochemical relationships
Autor/es:
  • Paz Ares, Javier
  • Hernandez Lucas, Carlos
  • Salcedo Duran, Gabriel
  • Aragoncillo Ballesteros, Cipriano
  • Ponz Ascaso, Fernando
  • García Olmedo, Francisco
Tipo de Documento: Artículo
Título de Revista/Publicación: Journal of Experimental Botany
Fecha: Abril 1983
Volumen: 34
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

The CM-proteins, which are salt-soluble proteins that can be extracted with chloroform: methanol (2: 1, v/v), seem to be present in the endosperm of all the cereal species investigated. Antibodies raised against a mixture of the barley CM-proteins (A-H) cross-reacted with wheat and rye proteins in Ouchterlony tests and a detailed study was carried out for purified wheat (CM1, CM2. CM3. CM3') and barley (CMa, CMb, CMc, CMd) CM-proteins. [35Sl-Cysteine-labelled endosperm proteins from wheat and barley were investigated by immuno-precipitation, electrophoresis and fluorography using the antibodies (A-H) and also those to a mixture of wheat CM-proteins and to CMd. There was complete antigenic identity for all the wheat proteins and CMd, some of the other proteins showed partial antigenic identity. Previously proposed genetic and biochemical relationships among these proteins were confirmed in the present study.

Más información

ID de Registro: 5939
Identificador DC: http://oa.upm.es/5939/
Identificador OAI: oai:oa.upm.es:5939
Identificador DOI: 10.1093/jxb/34.4.388
URL Oficial: http://jxb.oxfordjournals.org/content/34/4/388.short
Depositado por: Memoria Investigacion
Depositado el: 09 Feb 2011 08:42
Ultima Modificación: 20 Abr 2016 14:37
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