The CM-proteins from cereal endosperm: Immunochemical relationships

Paz Ares, Javier and Hernandez Lucas, Carlos and Salcedo Duran, Gabriel and Aragoncillo Ballesteros, Cipriano and Ponz Ascaso, Fernando and García Olmedo, Francisco (1983). The CM-proteins from cereal endosperm: Immunochemical relationships. "Journal of Experimental Botany", v. 34 (n. 4); pp. 388-395. ISSN 0022-0957. https://doi.org/10.1093/jxb/34.4.388.

Description

Title: The CM-proteins from cereal endosperm: Immunochemical relationships
Author/s:
  • Paz Ares, Javier
  • Hernandez Lucas, Carlos
  • Salcedo Duran, Gabriel
  • Aragoncillo Ballesteros, Cipriano
  • Ponz Ascaso, Fernando
  • García Olmedo, Francisco
Item Type: Article
Título de Revista/Publicación: Journal of Experimental Botany
Date: April 1983
ISSN: 0022-0957
Volume: 34
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The CM-proteins, which are salt-soluble proteins that can be extracted with chloroform: methanol (2: 1, v/v), seem to be present in the endosperm of all the cereal species investigated. Antibodies raised against a mixture of the barley CM-proteins (A-H) cross-reacted with wheat and rye proteins in Ouchterlony tests and a detailed study was carried out for purified wheat (CM1, CM2. CM3. CM3') and barley (CMa, CMb, CMc, CMd) CM-proteins. [35Sl-Cysteine-labelled endosperm proteins from wheat and barley were investigated by immuno-precipitation, electrophoresis and fluorography using the antibodies (A-H) and also those to a mixture of wheat CM-proteins and to CMd. There was complete antigenic identity for all the wheat proteins and CMd, some of the other proteins showed partial antigenic identity. Previously proposed genetic and biochemical relationships among these proteins were confirmed in the present study.

More information

Item ID: 5939
DC Identifier: http://oa.upm.es/5939/
OAI Identifier: oai:oa.upm.es:5939
DOI: 10.1093/jxb/34.4.388
Official URL: http://jxb.oxfordjournals.org/content/34/4/388.short
Deposited by: Memoria Investigacion
Deposited on: 09 Feb 2011 08:42
Last Modified: 20 Apr 2016 14:37
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