Susceptibility of phytopathogenic bacteria to wheat purothionins in vitro

Fernandez de Caleya, R. and Gonzalez Pascual, Blanca and García Olmedo, Francisco and Carbonero Zalduegui, Pilar (1972). Susceptibility of phytopathogenic bacteria to wheat purothionins in vitro. "Applied and Enviromental Microbiology", v. 23 (n. 5); pp. 998-1000.

Description

Title: Susceptibility of phytopathogenic bacteria to wheat purothionins in vitro
Author/s:
  • Fernandez de Caleya, R.
  • Gonzalez Pascual, Blanca
  • García Olmedo, Francisco
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Applied and Enviromental Microbiology
Date: May 1972
Volume: 23
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Purothionins are basic polypeptides with antimicrobial properties that are present in the endosperm of wheat and other cereal species. Susceptibility to wheat purothionins among phytopathogenic bacteria of the genera Pseudomonas, Xanthomonas, Agrobacterium, Erwinia, and Corynebacterium has been investigated. Sensitive strains have been found in all of these genera except Agrobacterium (the only strain of A. tumefaciens available proved to be resistant). Minimal inhibitory concentrations (MIC) with partially purified crude purothionins ranged from 1 fig/xn\ for C. sepedonicum (C.5) to 540 yug/ml for E. amylouora (E.3). Minimal bactericidal concentrations (MBC) were not higher than twice the MIC valué, except for C. poinsettiae (C.4) (MBC/MIC = 8). Purothionins a and /?, obtained by carboxymethyl-cellulose column chromatography, were tested against P. solanacearum (P.2) and X. phaseoli (X.2); a purothionin was more active than (3 against X.2, and fü more active than a against P.2. This suggests a relationship between polypeptide sequence and specificity of action.

More information

Item ID: 5987
DC Identifier: http://oa.upm.es/5987/
OAI Identifier: oai:oa.upm.es:5987
Official URL: http://aem.asm.org/content/vol23/issue5/index.dtl
Deposited by: Memoria Investigacion
Deposited on: 14 Feb 2011 09:37
Last Modified: 20 Apr 2016 14:39
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