Susceptibility of phytopathogenic bacteria to wheat purothionins in vitro

Fernandez de Caleya, R.; Gonzalez Pascual, Blanca; García Olmedo, Francisco y Carbonero Zalduegui, Pilar (1972). Susceptibility of phytopathogenic bacteria to wheat purothionins in vitro. "Applied and Enviromental Microbiology", v. 23 (n. 5); pp. 998-1000.

Descripción

Título: Susceptibility of phytopathogenic bacteria to wheat purothionins in vitro
Autor/es:
  • Fernandez de Caleya, R.
  • Gonzalez Pascual, Blanca
  • García Olmedo, Francisco
  • Carbonero Zalduegui, Pilar
Tipo de Documento: Artículo
Título de Revista/Publicación: Applied and Enviromental Microbiology
Fecha: Mayo 1972
Volumen: 23
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Purothionins are basic polypeptides with antimicrobial properties that are present in the endosperm of wheat and other cereal species. Susceptibility to wheat purothionins among phytopathogenic bacteria of the genera Pseudomonas, Xanthomonas, Agrobacterium, Erwinia, and Corynebacterium has been investigated. Sensitive strains have been found in all of these genera except Agrobacterium (the only strain of A. tumefaciens available proved to be resistant). Minimal inhibitory concentrations (MIC) with partially purified crude purothionins ranged from 1 fig/xn\ for C. sepedonicum (C.5) to 540 yug/ml for E. amylouora (E.3). Minimal bactericidal concentrations (MBC) were not higher than twice the MIC valué, except for C. poinsettiae (C.4) (MBC/MIC = 8). Purothionins a and /?, obtained by carboxymethyl-cellulose column chromatography, were tested against P. solanacearum (P.2) and X. phaseoli (X.2); a purothionin was more active than (3 against X.2, and fü more active than a against P.2. This suggests a relationship between polypeptide sequence and specificity of action.

Más información

ID de Registro: 5987
Identificador DC: http://oa.upm.es/5987/
Identificador OAI: oai:oa.upm.es:5987
URL Oficial: http://aem.asm.org/content/vol23/issue5/index.dtl
Depositado por: Memoria Investigacion
Depositado el: 14 Feb 2011 09:37
Ultima Modificación: 20 Abr 2016 14:39
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