Two cold inducible genes encoding lipid transfer protein LTP4 from barley show differential responses to bacterial pathogens

Molina Fernández, Antonio; Diaz Rodriguez, Isabel; Carbonero Zalduegui, Pilar; García Olmedo, Francisco y Vasil, Indra K. (1996). Two cold inducible genes encoding lipid transfer protein LTP4 from barley show differential responses to bacterial pathogens. "Molecular and General Genetics MGG", v. 252 (n. 1-2); pp. 162-168. ISSN 0026-8925. https://doi.org/10.1007/BF02173216.

Descripción

Título: Two cold inducible genes encoding lipid transfer protein LTP4 from barley show differential responses to bacterial pathogens
Autor/es:
  • Molina Fernández, Antonio
  • Diaz Rodriguez, Isabel
  • Carbonero Zalduegui, Pilar
  • García Olmedo, Francisco
  • Vasil, Indra K.
Tipo de Documento: Artículo
Título de Revista/Publicación: Molecular and General Genetics MGG
Fecha: Agosto 1996
Volumen: 252
Materias:
Palabras Clave Informales: Bacterial pathogens - Barley - Lipid transfer proteins - Low temperature - Promoter analysis
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

The barley genesHvLtp4.2 andHvLtp4.3 both encode the lipid transfer protein LTP4 and are less than 1 kb apart in tail-to-tail orientation. They differ in their non-coding regions from each other and from the gene corresponding to a previously reportedLtp4 cDNA (nowLtp4.1). Southern blot analysis indicated the existence of three or moreLtp4 genes per haploid genome and showed considerable polymorphism among barley cultivars. We have investigated the transient expression of genesHvLtp4.2 andHvLtp4.3 following transformation by particle bombardment, using promoter fusions to the-glucuronidase reporter sequence. In leaves, activities of the two promoters were of the same order as those of the sucrose synthase (Ss1) and cauliflower mosaic virus 35S promoters used as controls. Their expression patterns were similar, except thatLtp4.2 was more active thanLtp4.3 in endosperm, andLtp4.3 was active in roots, whileLtp4.2 was not. The promoters of both genes were induced by low temperature, both in winter and spring barley cultivars. Northern blot analysis, using theLtp4-specific probe, indicated thatXanthomonas campestris pv.translucens induced an increase over basal levels ofLtp4 mRNA, whilePseudomonas syringae pv.japonica caused a decrease. TheLtp4.3-Gus promoter fusion also responded in opposite ways to these two compatible bacterial pathogens, whereas theLtp4.2-Gus construction did not respond to infection

Más información

ID de Registro: 6020
Identificador DC: http://oa.upm.es/6020/
Identificador OAI: oai:oa.upm.es:6020
Identificador DOI: 10.1007/BF02173216
URL Oficial: http://www.springerlink.com/content/j00r300377487121/
Depositado por: Memoria Investigacion
Depositado el: 16 Feb 2011 09:09
Ultima Modificación: 20 Abr 2016 14:40
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