Two cold inducible genes encoding lipid transfer protein LTP4 from barley show differential responses to bacterial pathogens

Molina Fernández, Antonio and Diaz Rodriguez, Isabel and Carbonero Zalduegui, Pilar and García Olmedo, Francisco and Vasil, Indra K. (1996). Two cold inducible genes encoding lipid transfer protein LTP4 from barley show differential responses to bacterial pathogens. "Molecular and General Genetics MGG", v. 252 (n. 1-2); pp. 162-168. ISSN 0026-8925. https://doi.org/10.1007/BF02173216.

Description

Title: Two cold inducible genes encoding lipid transfer protein LTP4 from barley show differential responses to bacterial pathogens
Author/s:
  • Molina Fernández, Antonio
  • Diaz Rodriguez, Isabel
  • Carbonero Zalduegui, Pilar
  • García Olmedo, Francisco
  • Vasil, Indra K.
Item Type: Article
Título de Revista/Publicación: Molecular and General Genetics MGG
Date: August 1996
ISSN: 0026-8925
Volume: 252
Subjects:
Freetext Keywords: Bacterial pathogens - Barley - Lipid transfer proteins - Low temperature - Promoter analysis
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The barley genesHvLtp4.2 andHvLtp4.3 both encode the lipid transfer protein LTP4 and are less than 1 kb apart in tail-to-tail orientation. They differ in their non-coding regions from each other and from the gene corresponding to a previously reportedLtp4 cDNA (nowLtp4.1). Southern blot analysis indicated the existence of three or moreLtp4 genes per haploid genome and showed considerable polymorphism among barley cultivars. We have investigated the transient expression of genesHvLtp4.2 andHvLtp4.3 following transformation by particle bombardment, using promoter fusions to the-glucuronidase reporter sequence. In leaves, activities of the two promoters were of the same order as those of the sucrose synthase (Ss1) and cauliflower mosaic virus 35S promoters used as controls. Their expression patterns were similar, except thatLtp4.2 was more active thanLtp4.3 in endosperm, andLtp4.3 was active in roots, whileLtp4.2 was not. The promoters of both genes were induced by low temperature, both in winter and spring barley cultivars. Northern blot analysis, using theLtp4-specific probe, indicated thatXanthomonas campestris pv.translucens induced an increase over basal levels ofLtp4 mRNA, whilePseudomonas syringae pv.japonica caused a decrease. TheLtp4.3-Gus promoter fusion also responded in opposite ways to these two compatible bacterial pathogens, whereas theLtp4.2-Gus construction did not respond to infection

More information

Item ID: 6020
DC Identifier: http://oa.upm.es/6020/
OAI Identifier: oai:oa.upm.es:6020
DOI: 10.1007/BF02173216
Official URL: http://www.springerlink.com/content/j00r300377487121/
Deposited by: Memoria Investigacion
Deposited on: 16 Feb 2011 09:09
Last Modified: 20 Apr 2016 14:40
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