Expression of the alpha-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens

Carmona Quiles, Maria Jose and Molina Fernández, Antonio and Fernandez, Jose A. and Lopez Fando, J.J. and García Olmedo, Francisco (1993). Expression of the alpha-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens. "Plant Journal", v. 3 (n. 3); pp. 457-462. ISSN 0960-7412. https://doi.org/10.1046/j.1365-313X.1993.t01-24-00999.x.

Description

Title: Expression of the alpha-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens
Author/s:
  • Carmona Quiles, Maria Jose
  • Molina Fernández, Antonio
  • Fernandez, Jose A.
  • Lopez Fando, J.J.
  • García Olmedo, Francisco
Item Type: Article
Título de Revista/Publicación: Plant Journal
Date: March 1993
ISSN: 0960-7412
Volume: 3
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Thionins are cysteine-rich, 5 kDa polypeptides which are toxic to plant pathogens in vitro. Expression of the gene encoding alpha-thionin from barley endosperm, under the 35S promoter from cauliflower mosaic virus, conferred to transgenic tobacco enhanced resistance to the bacterial plant pathogens Pseudomonas syringae pv. tabaci 153 and P. syringae pv. syringae. The barley alpha-thionin gene, which has two introns, was correctly spliced in tobacco. The alpha-thionin in transgenic plants had the expected mobility in the gradient, when separated by high- performance liquid chromatography, reacted with monospecific antibodies and showed the expected antibiotic properties in vitro

More information

Item ID: 6032
DC Identifier: http://oa.upm.es/6032/
OAI Identifier: oai:oa.upm.es:6032
DOI: 10.1046/j.1365-313X.1993.t01-24-00999.x
Official URL: http://onlinelibrary.wiley.com/doi/10.1046/j.1365-313X.1993.t01-24-00999.x/abstract
Deposited by: Memoria Investigacion
Deposited on: 16 Feb 2011 12:03
Last Modified: 20 Apr 2016 14:40
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