Cloning of cDNA and chromosomal location of genes encoding the three types of subunits of the wheat tetrameric inhibitor of insect a-amylase

Garcia Maroto, Federico; Maraña, Carmen; Mena, M.; García Olmedo, Francisco y Carbonero Zalduegui, Pilar (1990). Cloning of cDNA and chromosomal location of genes encoding the three types of subunits of the wheat tetrameric inhibitor of insect a-amylase. "Plant Molecular Biology", v. 14 (n. 5); pp. 845-853. ISSN 0167-4412. https://doi.org/10.1007/BF00016517.

Descripción

Título: Cloning of cDNA and chromosomal location of genes encoding the three types of subunits of the wheat tetrameric inhibitor of insect a-amylase
Autor/es:
  • Garcia Maroto, Federico
  • Maraña, Carmen
  • Mena, M.
  • García Olmedo, Francisco
  • Carbonero Zalduegui, Pilar
Tipo de Documento: Artículo
Título de Revista/Publicación: Plant Molecular Biology
Fecha: Mayo 1990
Volumen: 14
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

Texto completo

[img]
Vista Previa
PDF (Document Portable Format) - Se necesita un visor de ficheros PDF, como GSview, Xpdf o Adobe Acrobat Reader
Descargar (375kB) | Vista Previa

Resumen

We have characterized three cDNA clones corresponding to proteins CM1, CM3 and CM16, which represent the three types of subunits of the wheat tetrameric inhibitor of insect -amylases. The deduced amino acid sequences of the mature polypeptides are homologous to those of the dimeric and monomeric -amylase inhibitors and of the trypsin inhibitors. The mature polypeptides are preceded by typical signal peptides. Southern blot analysis of appropriate aneuploids, using the cloned cDNAs as probes, has revealed the location of genes for subunits of the CM3 and of the CM16 type within a few kb of each other in chromosomes 4A, 4B and 4D, and those for the CM1 type of subunit in chromosomes 7A, 7B and 7D. Known subunits of the tetrameric inhibitor corresponding to genes from the B and D genomes have been previously characterized. No proteins of this class have been found to be encoded by the A genome in hexaploid wheat (genomes AA, BB, DD) or in diploid wheats (AA) and no anti -amylase activity has been detected in the latter, so that the A-genome genes must be either silent (pseudogenes) or expressed at a much lower level.

Más información

ID de Registro: 6042
Identificador DC: http://oa.upm.es/6042/
Identificador OAI: oai:oa.upm.es:6042
Identificador DOI: 10.1007/BF00016517
URL Oficial: http://www.springerlink.com/content/h1890w77g4626r24/
Depositado por: Memoria Investigacion
Depositado el: 16 Feb 2011 16:03
Ultima Modificación: 20 Abr 2016 14:41
  • Open Access
  • Open Access
  • Sherpa-Romeo
    Compruebe si la revista anglosajona en la que ha publicado un artículo permite también su publicación en abierto.
  • Dulcinea
    Compruebe si la revista española en la que ha publicado un artículo permite también su publicación en abierto.
  • Recolecta
  • e-ciencia
  • Observatorio I+D+i UPM
  • OpenCourseWare UPM