Cloning of cDNA and chromosomal location of genes encoding the three types of subunits of the wheat tetrameric inhibitor of insect a-amylase

Garcia Maroto, Federico and Maraña, Carmen and Mena, M. and García Olmedo, Francisco and Carbonero Zalduegui, Pilar (1990). Cloning of cDNA and chromosomal location of genes encoding the three types of subunits of the wheat tetrameric inhibitor of insect a-amylase. "Plant Molecular Biology", v. 14 (n. 5); pp. 845-853. ISSN 0167-4412. https://doi.org/10.1007/BF00016517.

Description

Title: Cloning of cDNA and chromosomal location of genes encoding the three types of subunits of the wheat tetrameric inhibitor of insect a-amylase
Author/s:
  • Garcia Maroto, Federico
  • Maraña, Carmen
  • Mena, M.
  • García Olmedo, Francisco
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Plant Molecular Biology
Date: May 1990
ISSN: 0167-4412
Volume: 14
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

We have characterized three cDNA clones corresponding to proteins CM1, CM3 and CM16, which represent the three types of subunits of the wheat tetrameric inhibitor of insect -amylases. The deduced amino acid sequences of the mature polypeptides are homologous to those of the dimeric and monomeric -amylase inhibitors and of the trypsin inhibitors. The mature polypeptides are preceded by typical signal peptides. Southern blot analysis of appropriate aneuploids, using the cloned cDNAs as probes, has revealed the location of genes for subunits of the CM3 and of the CM16 type within a few kb of each other in chromosomes 4A, 4B and 4D, and those for the CM1 type of subunit in chromosomes 7A, 7B and 7D. Known subunits of the tetrameric inhibitor corresponding to genes from the B and D genomes have been previously characterized. No proteins of this class have been found to be encoded by the A genome in hexaploid wheat (genomes AA, BB, DD) or in diploid wheats (AA) and no anti -amylase activity has been detected in the latter, so that the A-genome genes must be either silent (pseudogenes) or expressed at a much lower level.

More information

Item ID: 6042
DC Identifier: http://oa.upm.es/6042/
OAI Identifier: oai:oa.upm.es:6042
DOI: 10.1007/BF00016517
Official URL: http://www.springerlink.com/content/h1890w77g4626r24/
Deposited by: Memoria Investigacion
Deposited on: 16 Feb 2011 16:03
Last Modified: 20 Apr 2016 14:41
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