In vivo and in vitro synthesis of CM-proteins (A-hordeins) from barley (Hordeum vulgare L.)

Paz Ares, Javier and Ponz Ascaso, Fernando and Aragoncillo Ballesteros, Cipriano and Hernandez Lucas, Carlos and Salcedo Duran, Gabriel and Carbonero Zalduegui, Pilar and García Olmedo, Francisco (1983). In vivo and in vitro synthesis of CM-proteins (A-hordeins) from barley (Hordeum vulgare L.). "Planta", v. 157 (n. 1); pp. 74-80. ISSN 0032-0935. https://doi.org/10.1007/BF00394543.

Description

Title: In vivo and in vitro synthesis of CM-proteins (A-hordeins) from barley (Hordeum vulgare L.)
Author/s:
  • Paz Ares, Javier
  • Ponz Ascaso, Fernando
  • Aragoncillo Ballesteros, Cipriano
  • Hernandez Lucas, Carlos
  • Salcedo Duran, Gabriel
  • Carbonero Zalduegui, Pilar
  • García Olmedo, Francisco
Item Type: Article
Título de Revista/Publicación: Planta
Date: February 1983
ISSN: 0032-0935
Volume: 157
Subjects:
Freetext Keywords: A-hordein - CM-proteins - Endosperm - Hordeum (A-hordeins) - Protein biosynthesis
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

CM-proteins from barley endosperm (CMa, CMb, CMc, CMd), which are the main components of the A-hordein fraction, are synthesized most actively 10 to 30 d after anthesis (maximum at 15–20 d). They are synthesized by membranebound polysomes as precursors of higher apparent molecular weight (13,000–21,000) than the mature proteins (12,000–16,000). The largest in vitro product (21,000) is the putative precursor of protein CMd (16,000), as it is selected with anti-CMd monospecific IgG's, and is coded by an mRNA of greater sedimentation coefficient (9 S) than those encoding the other three proteins (7.5 S). CM-proteins always appear in the soluble fraction, following different homogenization and subcellular fractionation procedures, indicating that these proteins are transferred to the soluble fraction after processing.

More information

Item ID: 6075
DC Identifier: http://oa.upm.es/6075/
OAI Identifier: oai:oa.upm.es:6075
DOI: 10.1007/BF00394543
Official URL: http://www.springerlink.com/content/x1u50241w5p18p7q/
Deposited by: Memoria Investigacion
Deposited on: 18 Feb 2011 08:59
Last Modified: 20 Apr 2016 14:42
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