Paz Ares, Javier and Ponz Ascaso, Fernando and Aragoncillo Ballesteros, Cipriano and Hernandez Lucas, Carlos and Salcedo Duran, Gabriel and Carbonero Zalduegui, Pilar and García Olmedo, Francisco
In vivo and in vitro synthesis of CM-proteins (A-hordeins) from barley (Hordeum vulgare L.).
"Planta", v. 157
CM-proteins from barley endosperm (CMa, CMb, CMc, CMd), which are the main components of the A-hordein fraction, are synthesized most actively 10 to 30 d after anthesis (maximum at 15–20 d). They are synthesized by membranebound polysomes as precursors of higher apparent molecular weight (13,000–21,000) than the mature proteins (12,000–16,000). The largest in vitro product (21,000) is the putative precursor of protein CMd (16,000), as it is selected with anti-CMd monospecific IgG's, and is coded by an mRNA of greater sedimentation coefficient (9 S) than those encoding the other three proteins (7.5 S). CM-proteins always appear in the soluble fraction, following different homogenization and subcellular fractionation procedures, indicating that these proteins are transferred to the soluble fraction after processing.