CmVPS41 Is a General Gatekeeper for Resistance to Cucumber Mosaic Virus Phloem Entry in Melon

Pascual Bañuls, Laura and Yan, Jinqiang and Pujol, Marta and Monforte, Antonio J. and Picó, Belén and Montserrat Martín-Hernández, Ana (2019). CmVPS41 Is a General Gatekeeper for Resistance to Cucumber Mosaic Virus Phloem Entry in Melon. "Frontiers in Plant Science", v. 10 ; p. 1219. ISSN 1664-462X. https://doi.org/10.3389/fpls.2019.01219.

Description

Title: CmVPS41 Is a General Gatekeeper for Resistance to Cucumber Mosaic Virus Phloem Entry in Melon
Author/s:
  • Pascual Bañuls, Laura
  • Yan, Jinqiang
  • Pujol, Marta
  • Monforte, Antonio J.
  • Picó, Belén
  • Montserrat Martín-Hernández, Ana
Item Type: Article
Título de Revista/Publicación: Frontiers in Plant Science
Date: October 2019
ISSN: 1664-462X
Volume: 10
Subjects:
Freetext Keywords: melon; genetic diversity; VPS41; Cucumber mosaic virus; Resistance; Phloem loading
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Melon production is often compromised by viral diseases, which cannot be treated with chemicals. Therefore, the use of genetic resistances is the main strategy for generating crops resistant to viruses. Resistance to Cucumber mosaic virus (CMV) in melon is scarcely described in few accessions. Until recently, the only known resistant accessions were Freeman’s Cucumber and PI 161375, cultivar Songwhan Charmi (SC). Resistance to CMV in melon is recessive and generally oligogenic and quantitative. However, in SC, the resistance to CMV strains of subgroup II is monogenic, depending only on one gene, cmv1, which is able to stop CMV movement by restricting the virus to the bundle sheath cells and preventing a systemic infection. This restriction depends on the viral movement protein (MP). Chimeric viruses carrying the MP of subgroup II strains, like the strain LS (CMV-LS), are restricted in the bundle sheath cells, whereas those carrying MP from subgroup I, like the strain FNY (CMV-FNY), are able to overcome this restriction. cmv1encodes a vacuolar protein sorting 41 (CmVPS41), a protein involved in the transport of cargo proteins from the Golgi to the vacuole through late endosomes. We have analyzed the variability of the gene CmVPS41 in a set of 52 melon accessions belonging to 15 melon groups, both from the spp melo and the spp agrestis. We have identified 16 different haplotypes, encoding 12 different CmVPS41 protein variants. Challenging members of all haplotypes with CMV-LS, we have identified nine new resistant accessions. The resistance correlates with the presence of two mutations, either L348R, previously found in the accession SC and present in other three melon genotypes, or G85E, present in Freeman’s Cucumber and found also in four additional melon genotypes. Moreover, the new resistant accessions belong to three different melon horticultural groups, Conomon, Makuwa, and Dudaim. In the new resistant accessions, the virus was able to replicate and move cell to cell, but was not able to reach the phloem. Therefore, resistance to phloem entry seems to be a general strategy in melon controlled by CmVPS41. Finally, the newly reported resistant accessions broaden the possibilities for the use of genetic resistances in new melon breeding strategies.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL2012-40130-C02-01UnspecifiedUnspecifiedDisección genética de caracteres de interés agronómico en melón: resistencia a CMV y maduración climatérica de fruto
Government of SpainAGL2015-64625-C2-1-RUnspecifiedUnspecifiedDisección genética de dos caracteres de interés agronómico en melón: resistencia a cucumber mosaic virus y maduración climatérica de fruto
Government of SpainSEV‐2015‐0533UnspecifiedUnspecifiedUnspecified
Government of SpainAGL2015-64625-C2-2-RUnspecifiedUnspecifiedEvolución y diversificación en cucumis. Genética de la domesticación, morfología de fruto y barreras reproductivas
Government of SpainAGL2017-85563-C2-1-RUnspecifiedUnspecifiedControl multidisciplinar de enfermedades fúngicas y virosis en melón y sandía: un nuevo reto

More information

Item ID: 63254
DC Identifier: http://oa.upm.es/63254/
OAI Identifier: oai:oa.upm.es:63254
DOI: 10.3389/fpls.2019.01219
Official URL: http://doi.org/10.3389/fpls.2019.01219
Deposited by: Memoria Investigacion
Deposited on: 18 Sep 2020 10:02
Last Modified: 18 Sep 2020 10:02
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