Full text
|
PDF
- Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (2MB) | Preview |
Calero Muñoz, Nieves and Exposito Rodríguez, Marino and Collado Arenal, Aurelio M. and Rodríguez Serrano, María and Laureano Marín, Ana M. and Santamaría Fernández, María Estrella and Gotor, Cecilia and Diaz Rodriguez, Isabel and Mullineaux, Philip M. and Romero Puertas, María C. and Olmedilla, Adela and Sandalio, Luisa M. (2019). Cadmium induces ROS-dependent pexophagy in Arabidopsis leaves. "Plant Cell and Environment", v. 42 (n. 9); pp. 2696-2714. ISSN 1365-3040. https://doi.org/10.1111/pce.13597.
Title: | Cadmium induces ROS-dependent pexophagy in Arabidopsis leaves |
---|---|
Author/s: |
|
Item Type: | Article |
Título de Revista/Publicación: | Plant Cell and Environment |
Date: | September 2019 |
ISSN: | 1365-3040 |
Volume: | 42 |
Subjects: | |
Freetext Keywords: | ATG8; cadmium; caspase; catalase; cathepsin; legumain; NBR1; peroxisomes; pexophagy; ROS |
Faculty: | E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM) |
Department: | Biotecnología - Biología Vegetal |
Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
|
PDF
- Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (2MB) | Preview |
Cadmium treatment induces transient peroxisome proliferation in Arabidopsis leaves. To determine whether this process is regulated by pexophagy and to identify the mechanisms involved, we analysed time course‐dependent changes in ATG8, an autophagy marker, and the accumulation of peroxisomal marker PEX14a. After 3 hr of Cd exposure, the transcript levels of ATG8h , ATG8c , a , and i were slightly up‐regulated and then returned to normal. ATG8 protein levels also increased after 3 hr of Cd treatment, although an opposite pattern was observed in PEX14. Arabidopsis lines expressing GFP‐ATG8a and CFP‐SKL enabled us to demonstrate the presence of pexophagic processes in leaves. The Cd‐dependent induction of pexophagy was demonstrated by the accumulation of peroxisomes in autophagy gene (ATG)‐related Arabidopsis knockout mutants atg5 and atg7 . We show that ATG8a colocalizes with catalase and NBR1 in the electron‐dense peroxisomal core, thus suggesting that NBR1 may be an autophagic receptor for peroxisomes, with catalase being possibly involved in targeting pexophagy. Protein carbonylation and peroxisomal redox state suggest that protein oxidation may trigger pexophagy. Cathepsine B, legumain, and caspase 6 may also be involved in the regulation of pexophagy. Our results suggest that pexophagy could be an important step in rapid cell responses to cadmium.
Type | Code | Acronym | Leader | Title |
---|---|---|---|---|
Government of Spain | BIO2015‐68957‐REDT | Unspecified | Unspecified | Regulación redox y estrés oxidativo y nitrosativo en plantas |
Government of Spain | BIO2015‐67657‐P | Unspecified | Unspecified | Función de las especies de oxígeno y nitrógeno reactivos (ROS/RNS) en la dinámica y señalización peroxisomal en respuesta al estrés |
Government of Spain | BIO2016‐76633‐P | Unspecified | Unspecified | Señalizacion intracelular en plantas mediada por sulfuro y cianuro |
Government of Spain | PGC2018‐098372‐B‐I00 | Unspecified | Unspecified | Señalización dependiente de peroxisomas en condiciones de estres: peroxulos y homeostasis peroxisomal |
Government of Spain | I‐LINK1247 | Unspecified | Unspecified | Real-time monitoring of basal H2O2 levels with peroxiredoxin-based probes for plant science research |
Item ID: | 63299 |
---|---|
DC Identifier: | http://oa.upm.es/63299/ |
OAI Identifier: | oai:oa.upm.es:63299 |
DOI: | 10.1111/pce.13597 |
Official URL: | https://doi.org/10.1111/pce.13597 |
Deposited by: | Memoria Investigacion |
Deposited on: | 16 Sep 2020 15:31 |
Last Modified: | 16 Sep 2020 15:31 |