Cadmium induces ROS-dependent pexophagy in Arabidopsis leaves

Calero Muñoz, Nieves and Exposito Rodríguez, Marino and Collado Arenal, Aurelio M. and Rodríguez Serrano, María and Laureano Marín, Ana M. and Santamaría Fernández, María Estrella and Gotor, Cecilia and Diaz Rodriguez, Isabel and Mullineaux, Philip M. and Romero Puertas, María C. and Olmedilla, Adela and Sandalio, Luisa M. (2019). Cadmium induces ROS-dependent pexophagy in Arabidopsis leaves. "Plant Cell and Environment", v. 42 (n. 9); pp. 2696-2714. ISSN 1365-3040. https://doi.org/10.1111/pce.13597.

Description

Title: Cadmium induces ROS-dependent pexophagy in Arabidopsis leaves
Author/s:
  • Calero Muñoz, Nieves
  • Exposito Rodríguez, Marino
  • Collado Arenal, Aurelio M.
  • Rodríguez Serrano, María
  • Laureano Marín, Ana M.
  • Santamaría Fernández, María Estrella
  • Gotor, Cecilia
  • Diaz Rodriguez, Isabel
  • Mullineaux, Philip M.
  • Romero Puertas, María C.
  • Olmedilla, Adela
  • Sandalio, Luisa M.
Item Type: Article
Título de Revista/Publicación: Plant Cell and Environment
Date: September 2019
ISSN: 1365-3040
Volume: 42
Subjects:
Freetext Keywords: ATG8; cadmium; caspase; catalase; cathepsin; legumain; NBR1; peroxisomes; pexophagy; ROS
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Cadmium treatment induces transient peroxisome proliferation in Arabidopsis leaves. To determine whether this process is regulated by pexophagy and to identify the mechanisms involved, we analysed time course‐dependent changes in ATG8, an autophagy marker, and the accumulation of peroxisomal marker PEX14a. After 3 hr of Cd exposure, the transcript levels of ATG8h , ATG8c , a , and i were slightly up‐regulated and then returned to normal. ATG8 protein levels also increased after 3 hr of Cd treatment, although an opposite pattern was observed in PEX14. Arabidopsis lines expressing GFP‐ATG8a and CFP‐SKL enabled us to demonstrate the presence of pexophagic processes in leaves. The Cd‐dependent induction of pexophagy was demonstrated by the accumulation of peroxisomes in autophagy gene (ATG)‐related Arabidopsis knockout mutants atg5 and atg7 . We show that ATG8a colocalizes with catalase and NBR1 in the electron‐dense peroxisomal core, thus suggesting that NBR1 may be an autophagic receptor for peroxisomes, with catalase being possibly involved in targeting pexophagy. Protein carbonylation and peroxisomal redox state suggest that protein oxidation may trigger pexophagy. Cathepsine B, legumain, and caspase 6 may also be involved in the regulation of pexophagy. Our results suggest that pexophagy could be an important step in rapid cell responses to cadmium.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO2015‐68957‐REDTUnspecifiedUnspecifiedRegulación redox y estrés oxidativo y nitrosativo en plantas
Government of SpainBIO2015‐67657‐PUnspecifiedUnspecifiedFunción de las especies de oxígeno y nitrógeno reactivos (ROS/RNS) en la dinámica y señalización peroxisomal en respuesta al estrés
Government of SpainBIO2016‐76633‐PUnspecifiedUnspecifiedSeñalizacion intracelular en plantas mediada por sulfuro y cianuro
Government of SpainPGC2018‐098372‐B‐I00UnspecifiedUnspecifiedSeñalización dependiente de peroxisomas en condiciones de estres: peroxulos y homeostasis peroxisomal
Government of SpainI‐LINK1247UnspecifiedUnspecifiedReal-time monitoring of basal H2O2 levels with peroxiredoxin-based probes for plant science research

More information

Item ID: 63299
DC Identifier: http://oa.upm.es/63299/
OAI Identifier: oai:oa.upm.es:63299
DOI: 10.1111/pce.13597
Official URL: https://doi.org/10.1111/pce.13597
Deposited by: Memoria Investigacion
Deposited on: 16 Sep 2020 15:31
Last Modified: 16 Sep 2020 15:31
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