Phytoglobins in the nuclei, cytoplasm, and chloroplasts modulate nitric oxide signalling and crosstalk with abscisic acid

Rubio Luna, María Carmen and Calvo Begueria, Laura and Díaz Mendoza, María Mercedes and Elhiti, Mohamed and Moore, Marten and Matamoros Galindo, Manuel and James, Euan K. and Diaz Rodriguez, Isabel and Pérez Rantomé, Carmen and Villar Rua, Irene and Sein Echaluce, Violeta C. and Helbestrup, Kim H. and Dietz, Karl Josef and Becana Ausejo, Manuel (2019). Phytoglobins in the nuclei, cytoplasm, and chloroplasts modulate nitric oxide signalling and crosstalk with abscisic acid. "The Plant Journal", v. 100 (n. 1); pp. 38-54. ISSN 1365-313X. https://doi.org/10.1111/tpj.14422.

Description

Title: Phytoglobins in the nuclei, cytoplasm, and chloroplasts modulate nitric oxide signalling and crosstalk with abscisic acid
Author/s:
  • Rubio Luna, María Carmen
  • Calvo Begueria, Laura
  • Díaz Mendoza, María Mercedes
  • Elhiti, Mohamed
  • Moore, Marten
  • Matamoros Galindo, Manuel
  • James, Euan K.
  • Diaz Rodriguez, Isabel
  • Pérez Rantomé, Carmen
  • Villar Rua, Irene
  • Sein Echaluce, Violeta C.
  • Helbestrup, Kim H.
  • Dietz, Karl Josef
  • Becana Ausejo, Manuel
Item Type: Article
Título de Revista/Publicación: The Plant Journal
Date: October 2019
ISSN: 1365-313X
Volume: 100
Subjects:
Freetext Keywords: bscisic acid; Arabidopsis thaliana; Lotus japonicus; nitric oxide; phytoglobins
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Symbiotic hemoglobins provide O2 to N2‐fixing bacteria within legume nodules, but the functions of non‐symbiotic hemoglobins or phytoglobins (Glbs) are much less defined. Immunolabeling combined with confocal microscopy of the Glbs tagged at the C‐terminus with green fluorescent protein was used to determine their subcellular localizations in Arabidopsis and Lotus japonicus . Recombinant proteins were used to examine nitric oxide (NO ) scavenging in vitro and transgenic plants to show S‐ nitrosylation and other in vivo interactions with NO and abscisic acid (ABA) responses. We found that Glbs occur in the nuclei, chloroplasts and amyloplasts of both model plants, and also in the cytoplasm of Arabidopsis cells. The proteins show similar NO dioxygenase activities in vitro , are nitrosylated in Cys residues in vivo , and scavenge NO in the stomatal cells. The Cys/Ser mutation does not affect NO dioxygenase activity, and S‐nitrosylation does not significantly consume NO . We demonstrate an interaction between Glbs and ABA on several grounds: Glb1 and Glb2 scavenge NO produced in stomatal guard cells following ABA supply; plants overexpressing Glb1 show higher constitutive expression of the ABA responsive genes Responsive to ABA (RAB 18 ), Responsive to Dehydration (RD 29A ) and Highly ABA ‐Induced 2 (HAI 2 ), and are more tolerant to dehydration; and ABA strongly upregulates class 1 Glbs. We conclude that Glbs modulate NO and interact with ABA in crucial physiological processes such as the plant's response to dessication.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL2014-53717-RUnspecifiedUnspecifiedFijación de nitrógeno por la simbiosis rizobio-leguminosa: hemoglobinas y modificaciones oxidativas de las proteínas durante el desarrollo y senescencia de los nódulos
Government of SpainAGL2017-85775-RUnspecifiedUnspecifiedFunción de las hemoglobinas y del óxido nítrico en la simbiosis rizobio-leguminosa

More information

Item ID: 63349
DC Identifier: http://oa.upm.es/63349/
OAI Identifier: oai:oa.upm.es:63349
DOI: 10.1111/tpj.14422
Official URL: https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.14422
Deposited by: Memoria Investigacion
Deposited on: 11 Nov 2020 08:12
Last Modified: 11 Nov 2020 08:12
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