Artemisia pollen is the main vector for airborne endotoxin

Oteros, Jose and Bartusel, Elke and Alessandrini, Francesca and Núñez, Andrés and Moreno Gómez, Diego Alejandro and Behrendt, Heidrun and Schmidt-Weber, Carsten and Traidl-Hoffmann, Claudia and Buters, Jeroen (2019). Artemisia pollen is the main vector for airborne endotoxin. "Journal of Allergy And Clinical Immunology", v. 143 (n. 1); pp.. ISSN 0091-6749. https://doi.org/10.1016/j.jaci.2018.05.040.

Description

Title: Artemisia pollen is the main vector for airborne endotoxin
Author/s:
  • Oteros, Jose
  • Bartusel, Elke
  • Alessandrini, Francesca
  • Núñez, Andrés
  • Moreno Gómez, Diego Alejandro
  • Behrendt, Heidrun
  • Schmidt-Weber, Carsten
  • Traidl-Hoffmann, Claudia
  • Buters, Jeroen
Item Type: Article
Título de Revista/Publicación: Journal of Allergy And Clinical Immunology
Date: January 2019
ISSN: 0091-6749
Volume: 143
Subjects:
Freetext Keywords: Endotoxin; ambient; bacteria; pollen; gram-negative; recombinant Factor c; Davos; Munich; Artemisia species; micro-biome; mouse model; allergy; LPS; source; PM10; PM2.5
Faculty: E.T.S.I. Industriales (UPM)
Department: Física Aplicada e Ingeniería de Materiales
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Background: Endotoxin (LPS) released from gram-negative bacteria causes strong immunologic and inflammatory effects and, when airborne, can contribute to respiratory conditions, such as allergic asthma. Objectives: We sought to identify the source of airborne endotoxin and the effect of this endotoxin on allergic sensitization. Methods: We determined LPS levels in outdoor air on a daily basis for 4 consecutive years in Munich (Germany) and Davos (Switzerland). Air was sampled as particulate matter (PM) greater than 10mm (PM > 10) and PM between 2.5 and 10mm. LPS levels were determined by using the recombinant Factor C assay. Results: More than 60% of the annual endotoxin exposure was detected in the PM > 10 fraction, showing that bacteria do not aerosolize as independent units or aggregates but adhered to large particles. In Munich 70% of annual exposure was detected between June 12th and August 28th. Multivariate modeling showed that endotoxin levels could be explained by phenological parameters (ie, plant growth). Indeed, days with high airborne endotoxin levels correlated well with the amount of Artemisia pollen in the air. Pollen collected from plants across Europe (100 locations) showed that the highest levels of endotoxin were detected on Artemisia vulgaris (mugwort) pollen, with little on other pollen. Microbiome analysis showed that LPS concentrations on mugwort pollen were related to the presence of Pseudomonas species and Pantoea species communities. In a mouse model of allergic disease, the presence of LPS on mugwort pollen was needed for allergic sensitization. Conclusions: The majority of airborne endotoxin stems from bacteria dispersed with pollen of only one plant: mugwort. This LPS was essential for inducing inflammation of the lung and allergic sensitization.

More information

Item ID: 63609
DC Identifier: http://oa.upm.es/63609/
OAI Identifier: oai:oa.upm.es:63609
DOI: 10.1016/j.jaci.2018.05.040
Official URL: https://www.sciencedirect.com/science/article/pii/S0091674918309990?via%3Dihub
Deposited by: Memoria Investigacion
Deposited on: 29 Sep 2020 15:09
Last Modified: 15 Oct 2020 16:04
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