Preventing legionellosis outbreaks by a quick detection of airborne Legionella pneumophila

Sánchez Parra, Beatriz and Núñez, Andrés and Moreno Gómez, Diego Alejandro (2019). Preventing legionellosis outbreaks by a quick detection of airborne Legionella pneumophila. "Environmental Research", v. 171 ; pp. 546-549. ISSN 0013-9351. https://doi.org/10.1016/j.envres.2019.01.032.

Description

Title: Preventing legionellosis outbreaks by a quick detection of airborne Legionella pneumophila
Author/s:
  • Sánchez Parra, Beatriz
  • Núñez, Andrés
  • Moreno Gómez, Diego Alejandro
Item Type: Article
Título de Revista/Publicación: Environmental Research
Date: May 2019
ISSN: 0013-9351
Volume: 171
Subjects:
Freetext Keywords: Legionella pneumophila; Epidemic; Bioaerosols; PCR; 16S rRNA
Faculty: E.T.S.I. Industriales (UPM)
Department: Física Aplicada e Ingeniería de Materiales
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Legionellosis is a severe pneumonic infection caused by inhaling bacteria of the genus Legionella. Most cases reported in the USA and Europe are associated with the species Legionella pneumophila. This Gram-negative bacterium can survive within a wide spectrum of temperatures, and be transmitted via aerosols from multiple aquatic sources: fountains, thermal spas and other water systems. Although the PCR is one of the most popular methods to verify its presence in environmental or clinical samples, the direct application of this technique to ambient air samples is unusual because of the scarce material in the specimens. Here, we have developed a two-PCR assay, carried out over the V3 and V5 hypervariable regions of the 16S rRNA gene, to detect specifically the pathogenic bacteria Legionella pneumophila in outdoor air samples with low concentration of DNA. The application of this protocol does not require culture and retrieves quick results to activate the corresponding public alerts to prevent legionellosis outbreaks.

Funding Projects

TypeCodeAcronymLeaderTitle
Madrid Regional Government2013/MAE-2874AIRBIOTA-CMUnspecifiedConocer y modelizar la contaminación biológica del aire urbano

More information

Item ID: 63610
DC Identifier: http://oa.upm.es/63610/
OAI Identifier: oai:oa.upm.es:63610
DOI: 10.1016/j.envres.2019.01.032
Official URL: https://www.sciencedirect.com/science/article/pii/S0013935119300325
Deposited by: Memoria Investigacion
Deposited on: 30 Sep 2020 12:20
Last Modified: 01 Jun 2021 22:30
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