Comprehensive analysis of different adhesives in aerobiological sampling using optical microscopy and high-throughput DNA sequencing

Rojo, Jesús and Nuñez Hernández, Andrés and Lara, Beatriz and Sánchez Parra, Beatriz and Moreno Gómez, Diego Alejandro and Pérez-Badia, Rosa (2019). Comprehensive analysis of different adhesives in aerobiological sampling using optical microscopy and high-throughput DNA sequencing. "Journal of Environmental Management", v. 240 ; pp. 441-450. ISSN 0301-4797. https://doi.org/10.1016/j.jenvman.2019.03.116.

Description

Title: Comprehensive analysis of different adhesives in aerobiological sampling using optical microscopy and high-throughput DNA sequencing
Author/s:
  • Rojo, Jesús
  • Nuñez Hernández, Andrés
  • Lara, Beatriz
  • Sánchez Parra, Beatriz
  • Moreno Gómez, Diego Alejandro
  • Pérez-Badia, Rosa
Item Type: Article
Título de Revista/Publicación: Journal of Environmental Management
Date: 15 June 2019
ISSN: 0301-4797
Volume: 240
Subjects:
Freetext Keywords: Aerobiology; Adhesive; Morphological analysis; High-throughput DNA sequencing; Pollen
Faculty: E.T.S.I. Industriales (UPM)
Department: Física Aplicada e Ingeniería de Materiales
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The standardization and unification of the procedures to analyze and quantify the airborne pollen concentrations are very important topics. In this work, the effectiveness of the two most used adhesives in aerobiological sampling, silicone prepared with cyclohexane solvent (Silicone) and petroleum jelly (Vaseline), was compared under outdoor conditions. This comparison was carried out using the traditional method based on the identification and quantification by optical microscopy (OM) of the airborne pollen and the novel methodology by high-throughput sequencing analysis (HTS). Globally, the results from both methods of analysis (OM and HTS) showed a good agreement between the two adhesives tested regarding the abundance of the main pollen types present in the samples: Cupressaceae, Olea, Poaceae, Platanus, Quercus. We concluded that the results from both adhesives are comparable data. Furthermore, the comparisons between methodologies, OM vs. HTS, showed that both techniques can accurately identify the most abundant pollen types in the atmosphere for the studied periods, with a good agreement of their relative abundances especially when the airborne pollen diversity is low but showing some divergences as the number of pollen types increases.

Funding Projects

TypeCodeAcronymLeaderTitle
Madrid Regional GovernmentS2013/MAE-2874AIRBIOTA-CMUnspecifiedConocer y modelizar la contaminación biológica del aire urbano

More information

Item ID: 63613
DC Identifier: http://oa.upm.es/63613/
OAI Identifier: oai:oa.upm.es:63613
DOI: 10.1016/j.jenvman.2019.03.116
Official URL: https://www.journals.elsevier.com/journal-of-environmental-management
Deposited by: Memoria Investigacion
Deposited on: 30 Sep 2020 13:04
Last Modified: 15 Oct 2020 16:27
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