Optimizing Small World Initiative service learning by focusing on antibiotics-producing actinomycetes from soil

De Groot, Piet and Fernández-Pereira, Jordan and Sabariegos, Rosario and Clemente-Casares, Pilar and Parra-Martínez, Javier and J. Cid, Víctor and Moreno Gómez, Diego Alejandro (2020). Optimizing Small World Initiative service learning by focusing on antibiotics-producing actinomycetes from soil. "FEMS Microbiology Letters", v. 366 (n. 24); pp. 1-8. ISSN 0378-1097. https://doi.org/10.1093/femsle/fnaa019.

Description

Title: Optimizing Small World Initiative service learning by focusing on antibiotics-producing actinomycetes from soil
Author/s:
  • De Groot, Piet
  • Fernández-Pereira, Jordan
  • Sabariegos, Rosario
  • Clemente-Casares, Pilar
  • Parra-Martínez, Javier
  • J. Cid, Víctor
  • Moreno Gómez, Diego Alejandro
Item Type: Article
Título de Revista/Publicación: FEMS Microbiology Letters
Date: 29 January 2020
ISSN: 0378-1097
Volume: 366
Subjects:
Freetext Keywords: Small World Initiative (SWI); Tiny Earth; citizen science; service learning; antibiotic awareness; actinomycetes
Faculty: E.T.S.I. Industriales (UPM)
Department: Física Aplicada e Ingeniería de Materiales
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Small World Initiative and Tiny Earth are popular citizen science programs that are implemented worldwide in response to the global antibiotic resistance crisis. When starting up the program in Albacete (Spain), we noted that rates of isolated antibiotic-producing bacteria are generally low. To make the activity more stimulating for participating students, we modified the protocol to obtain more positive results by focusing on isolation of actinomycetes, the main producers of most clinically used antibiotics. Adaptations involved redesigning culture media, incubation times and temperatures, and modification of the ESKAPE antibiosis experiment by employing an agar-transplantation step. Of 390 bacterial isolates tested, almost 6% tested positive in antibiosis experiments and DNA sequence analysis confirmed that all positives are actinomycetes, demonstrating that our protocol is efficient toward isolating antibiotic-producing actinomycetes from soil. Evaluation forms filled by participating students indicated that the program was received very positively and that our modifications contribute to make this educational program more stimulating and efficient.

More information

Item ID: 63615
DC Identifier: http://oa.upm.es/63615/
OAI Identifier: oai:oa.upm.es:63615
DOI: 10.1093/femsle/fnaa019
Official URL: https://academic.oup.com/femsle
Deposited by: Memoria Investigacion
Deposited on: 02 Oct 2020 16:11
Last Modified: 02 Oct 2020 16:11
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