ZP4 confers structural properties to the zona pellucida essential for embryo development

Lamas Toranzo, Ismael and Fonseca Baldís, N. and Querejeta Fernández, Ana and José Izquierdo-Rico, María and González Brusi, Leopoldo and Lorenzo González, Pedro Luis and Garcia Rebollar, Pilar and Avilés, Manuel and Bermejo Alvárez, Pablo (2019). ZP4 confers structural properties to the zona pellucida essential for embryo development. "eLife", v. 8 ; p. 48904. ISSN 2050-084X. https://doi.org/10.7554/eLife.48904.

Description

Title: ZP4 confers structural properties to the zona pellucida essential for embryo development
Author/s:
  • Lamas Toranzo, Ismael
  • Fonseca Baldís, N.
  • Querejeta Fernández, Ana
  • José Izquierdo-Rico, María
  • González Brusi, Leopoldo
  • Lorenzo González, Pedro Luis
  • Garcia Rebollar, Pilar
  • Avilés, Manuel
  • Bermejo Alvárez, Pablo
Item Type: Article
Título de Revista/Publicación: eLife
Date: October 2019
ISSN: 2050-084X
Volume: 8
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Producción Agraria
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (1MB) | Preview

Abstract

Zona pellucida (ZP), the extracellular matrix sheltering mammalian oocytes and embryos, is composed by 3 to 4 proteins. The roles of the three proteins present in mice have been elucidated by KO models, but the function of the fourth component (ZP4), present in all other eutherian mammals studied so far, has remained elusive. Herein, we report that ZP4 ablation impairs fertility in female rabbits. Ovulation, fertilization and in vitro development to blastocyst were not affected by ZP4 ablation. However, in vivo development is severely impaired in embryos covered by a ZP4-devoided zona, suggesting a defective ZP protective capacity in the absence of ZP4. ZP4-null ZP was significantly thinner, more permeable, and exhibited a more disorganized and fenestrated structure. The evolutionary conservation of ZP4 in other mammals, including humans, suggests that the structural properties conferred by this protein are required to ensure proper embryo sheltering during in vivo preimplantation development.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL2014-58739-RUnspecifiedUnspecifiedDesarrollo de la tecnología CRISPR para la modificación génica en especies de interés ganadero
Government of SpainRYC-2012-10193UnspecifiedUnspecifiedUnspecified
Government of SpainAGL2017-84908-RUnspecifiedUnspecifiedEstudio molecular de fallos reproductivos en especies ganaderas mediante la tecnología CRISPR
Government of SpainAGL2016-71890-REDTUnspecifiedUnspecifiedUnspecified
Government of SpainAGL2015-70159-PUnspecifiedUnspecifiedPapel de las secreciones del tracto genital femenino en la selección espermática y su posible implicación en la mejora de las técnicas de reproducción asistida
Government of SpainAGL2015-65572-C2-1-RUnspecifiedUnspecifiedUnspecified
Horizon 2020757886ELONGANInstituto Nacional de Investigación y Tecnología Agraria y Alimentaria OA MPGene editing and in vitro approaches to understand conceptus elongation in ungulates
FP7600391FELLOWSEAUniversidad de VigoProgramme for Post-Doctoral Talent Attraction to Campus do Mar, FELLOWSEA
Government of SpainPGC2018-094781-B-100UnspecifiedUnspecifiedEstudio de las implicaciones funcionales de proteínas ovocitarias y oviductales en la fecundación y desarrollo embrionario en la coneja mediante el uso de edición génica CRISP

More information

Item ID: 63659
DC Identifier: http://oa.upm.es/63659/
OAI Identifier: oai:oa.upm.es:63659
DOI: 10.7554/eLife.48904
Official URL: https://elifesciences.org/articles/48904
Deposited by: Memoria Investigacion
Deposited on: 16 Oct 2020 07:03
Last Modified: 16 Oct 2020 11:37
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM