Chemoperception of Specific Amino Acids Controls Phytopathogenicity in Pseudomonas syringae pv. tomato

Cerna Vargas, Jean Paul and Santamaría Hernándo, Saray and Matilla Vázquez, Miguel Ángel and Rodríguez Herva, Jose Juan and Daddaoua, Abdelali and Rodriguez Palenzuela, Pablo and Krell, Tino and Lopez Solanilla, Emilia (2019). Chemoperception of Specific Amino Acids Controls Phytopathogenicity in Pseudomonas syringae pv. tomato. "mBio", v. 10 (n. 5); pp.. ISSN 2150-7511. https://doi.org/10.1128/mBio.01868-19.

Description

Title: Chemoperception of Specific Amino Acids Controls Phytopathogenicity in Pseudomonas syringae pv. tomato
Author/s:
  • Cerna Vargas, Jean Paul
  • Santamaría Hernándo, Saray
  • Matilla Vázquez, Miguel Ángel
  • Rodríguez Herva, Jose Juan
  • Daddaoua, Abdelali
  • Rodriguez Palenzuela, Pablo
  • Krell, Tino
  • Lopez Solanilla, Emilia
Item Type: Article
Título de Revista/Publicación: mBio
Date: October 2019
ISSN: 2150-7511
Volume: 10
Subjects:
Freetext Keywords: chemoreceptors; Pseudomonas syringae; virulence
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Chemotaxis has been associated with the pathogenicity of bacteria in plants and was found to facilitate bacterial entry through stomata and wounds. However, knowledge regarding the plant signals involved in this process is scarce. We have addressed this issue using Pseudomonas syringae pv. tomato, which is a foliar pathogen that causes bacterial speck in tomato. We show that the chemoreceptor P. syringae pv. tomato PscA (PsPto-PscA) recognizes specifically and with high affinity l-Asp, l-Glu, and d-Asp. The mutation of the chemoreceptor gene largely reduced chemotaxis to these ligands but also altered cyclic di-GMP (c-di-GMP) levels, biofilm formation, and motility, pointing to cross talk between different chemosensory pathways. Furthermore, the PsPto-PscA mutant strain showed reduced virulence in tomato. Asp and Glu are the most abundant amino acids in plants and in particular in tomato apoplasts, and we hypothesize that this receptor may have evolved to specifically recognize these compounds to facilitate bacterial entry into the plant. Infection assays with the wild-type strain showed that the presence of saturating concentrations of d-Asp also reduced bacterial virulence.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainRTI2018-095222-B-I00UnspecifiedUnspecifiedRelevancia de la quimiopercepción durante el proceso de infección de bacterias fitopatógenas
Government of SpainGL2015-63851-RUnspecifiedUnspecifiedUnspecified
Government of SpainBIO2016-76779-PUnspecifiedUnspecifiedIdentificación sistemática y de alto rendimiento de moléculas señal reconocidas por proteínas sensoras bacterianas
Government of SpainBES-2016-076452UnspecifiedUnspecifiedFPI program

More information

Item ID: 63736
DC Identifier: http://oa.upm.es/63736/
OAI Identifier: oai:oa.upm.es:63736
DOI: 10.1128/mBio.01868-19
Official URL: https://mbio.asm.org/content/mbio/10/5/e01868-19.full.pdf
Deposited by: Memoria Investigacion
Deposited on: 17 Dec 2020 11:00
Last Modified: 17 Dec 2020 11:57
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