Direct growth of few-layer graphene on AlN-based resonators for high-sensitivity gravimetric biosensors

Olivares Roza, Jimena and Mirea, Teona and Gordillo Dagallier, Lorena and Marco Dufort, Bruno and Escolano Moyano, José Miguel and Clement Lorenzo, Marta and Iborra Grau, Enrique (2019). Direct growth of few-layer graphene on AlN-based resonators for high-sensitivity gravimetric biosensors. "Beilstein Journal of Nanotechnology", v. 10 ; pp. 975-984. ISSN 2190-4286. https://doi.org/10.3762/bjnano.10.98.

Description

Title: Direct growth of few-layer graphene on AlN-based resonators for high-sensitivity gravimetric biosensors
Author/s:
  • Olivares Roza, Jimena
  • Mirea, Teona
  • Gordillo Dagallier, Lorena
  • Marco Dufort, Bruno
  • Escolano Moyano, José Miguel
  • Clement Lorenzo, Marta
  • Iborra Grau, Enrique
Item Type: Article
Título de Revista/Publicación: Beilstein Journal of Nanotechnology
Date: April 2019
ISSN: 2190-4286
Volume: 10
Subjects:
Freetext Keywords: biomolecule detection; graphene integration; gravimetric biosensor; surface functionalization
Faculty: E.T.S.I. Telecomunicación (UPM)
Department: Ingeniería Electrónica
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

We present the successful growth of few-layer graphene on top of AlN-based solidly mounted resonators (SMR) using a low-temperature chemical vapour deposition (CVD) process assisted by Ni catalysts, and its effective bio-functionalization with antibodies. The SMRs are manufactured on top of fully insulating AlN/SiO2 acoustic mirrors able to withstand the temperatures reached during the CVD growth of graphene (up to 650 °C). The active AlN films, purposely grown with the c-axis tilted, effectively excite shear modes displaying excellent in-liquid performance, with electromechanical coupling and quality factors of around 3% and 150, respectively, which barely vary after graphene integration. Raman spectra reveal that the as-grown graphene is composed of less than five weakly coupled layers with a low density of defects. Two functionalization protocols of the graphene are proposed. The first one, based on a covalent binding approach, starts with a low-damage O2 plasma treatment that introduces a controlled density of defects in graphene, including carboxylic groups. After that, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) chemistry is used to covalently bind streptavidin molecules to the surface of the sensors. The second functionalization protocol is based on the non-covalent bonding of streptavidin on hydrophobic graphene surfaces. The two protocols end with the effective bonding of biotinylated anti-IgG antibodies to the streptavidin, which leaves the surface of the devices ready for possible IgG detection.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainMAT2013-45957-RUnspecifiedUnspecifiedMateriales avanzados para biosensores basados en resonadores piezoeléctricos combinados con nanotubos de carbono y grafeno

More information

Item ID: 64302
DC Identifier: http://oa.upm.es/64302/
OAI Identifier: oai:oa.upm.es:64302
DOI: 10.3762/bjnano.10.98
Official URL: https://www.beilstein-journals.org/bjnano/articles/10/98
Deposited by: Memoria Investigacion
Deposited on: 10 Oct 2020 11:18
Last Modified: 10 Oct 2020 11:18
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