Función de la proteasa CAPN12 en la regulación de la glucólisis y la polimerización de filamentos de vimentina en linfocitos T

Piñeiro Bugallo, Alexandra (2020). Función de la proteasa CAPN12 en la regulación de la glucólisis y la polimerización de filamentos de vimentina en linfocitos T. Proyecto Fin de Carrera / Trabajo Fin de Grado, E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM), Madrid.

Description

Title: Función de la proteasa CAPN12 en la regulación de la glucólisis y la polimerización de filamentos de vimentina en linfocitos T
Author/s:
  • Piñeiro Bugallo, Alexandra
Contributor/s:
  • Lacalle Blanco, Rosa Ana
  • Gonzalez-Melendi de Leon, Pablo
Item Type: Final Project
Degree: Grado en Biotecnología
Date: February 2020
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Calpains, a family of Ca2+ dependent cysteine-protease, are involved in many diseases such as cancer. This work has focused on the study of Calpain 12 (CAPN12), typical and with a predominant expression in skin and hair follicle. It is known their connection with cytoskeletal proteins, such as the vimentin. Vimentin is an intermediate filament involved in mechanical disruption, transendothelial migration, tumorigenesis and lymphocyte cell function. It has been studied CANP12 relation with vimentin cytoskeleton. The CAPN12 proteolytic capacity in vitro assays with vimentin filaments have shown a vimentin partial digestion. We have analysed how CAPN12, overexpression or genetic deletion, affects cell growth and cell glycolytic and mitochondrial metabolism in T lymphocytes. CAPN12 overexpression, in 2B4 cells, partially decreased cell proliferation and increased both the glycolytic and the mitochondrial respiration rates. We have also studied the CAPN12 relevance in the CD8+ effector function stimulated under conditions of activity and dysfunctionality, via PD-1. The metabolic studies performed with CD8+ from CAPN12 knockout (KO) mice showed an increase in the metabolic rate. These data suggest the CAPN12 as an anti-proliferative and as a negative metabolism regulator in T lymphocytes.

More information

Item ID: 64401
DC Identifier: http://oa.upm.es/64401/
OAI Identifier: oai:oa.upm.es:64401
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 06 Oct 2020 11:04
Last Modified: 06 Oct 2020 11:04
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