Papel de autofagia durante la transfagocitosis bacteriana en linfocitos B convencionales

Polo Picasso, Isabel (2020). Papel de autofagia durante la transfagocitosis bacteriana en linfocitos B convencionales. Proyecto Fin de Carrera / Trabajo Fin de Grado, E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM), Madrid.

Description

Title: Papel de autofagia durante la transfagocitosis bacteriana en linfocitos B convencionales
Author/s:
  • Polo Picasso, Isabel
Contributor/s:
  • Veiga Chacón, Esteban
  • Rodríguez Herva, Jose Juan
Item Type: Final Project
Degree: Grado en Biotecnología
Date: February 2020
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

It is well established that B cells are antigen presenting cells (APC). Indeed, it is known that, in vivo, B cells are able to uptake soluble and surface-tethered antigens presented on different types of cells. However, the way B cells capture entire bacteria remains unclear. The host group recently observed that B cells are able to capture bacteria by transphagocytosis from infected dendritic cells (DC) during the B cell/infected DC contacts. Furthermore, this transphagocytic B cells (trB cells) cross-present antigens (in the Major Histocompatibility Complex I, MHC-I) from captured L. monocytogenes, potently priming naive CD8+ T cells. This process was previously described for T CD4+ T cells. The present Final Degree Project aims to elucidate whether autophagy plays an important role in trB cells-mediated antigen cross-presentation, and whether autophagy is triggered by the route by which captured bacteria are degraded. The explicit objective is comparing the induction of autophagy in trB cells. This B cells captured different strains of L. monocytogenes, able or not to escape the phagosome reaching the cytosol, and Escherichia coli, not able to escape the phagosome. The aim is to determine whether bacterial escape into the cytosol would enhance autophagy and antigen cross-presentation. The present study showed that L. monocytogenes 10403S (WT) escaping the phagosome induce autophagy in trB cells, and listeriolysin O was the main responsible for this activation. Bacteria unable to escape into the cytosol, did not induce autophagy neither antigen crosspresentation.

More information

Item ID: 64416
DC Identifier: http://oa.upm.es/64416/
OAI Identifier: oai:oa.upm.es:64416
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 07 Oct 2020 08:08
Last Modified: 07 Oct 2020 08:08
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