Impact of b2-adrenergic receptor polymorphisms in heart failure: Cell surface expression and internalization

Imbert Griño, María (2020). Impact of b2-adrenergic receptor polymorphisms in heart failure: Cell surface expression and internalization. Proyecto Fin de Carrera / Trabajo Fin de Grado, E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM).

Description

Title: Impact of b2-adrenergic receptor polymorphisms in heart failure: Cell surface expression and internalization
Author/s:
  • Imbert Griño, María
Contributor/s:
  • Miedes Vicente, Eva
  • Pons, Véronique
Item Type: Final Project
Degree: Grado en Biotecnología
Date: 24 July 2020
Subjects:
Freetext Keywords: heart failure ; polymorphisms ; β2-Adrenergic receptor
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - Non commercial - Share

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Abstract

Heart Failure (HF) represents a major health problem throughout all over the world. One of the most cardiotoxic effects of HF is chronic catecholamine hypersecretion. In the heart, we can find β2-Adrenergic Receptors (β2-AR). β2-AR are known to be activated by catecholamines such as epinephrine and in consequence of this activation, receptors are internalized. It has been shown that certain polymorphisms of the β2-AR respond differently to ligand stimulation therefore being correlated with the severity of HF. These polymorphisms are: R16G, Q27E and T164I. We performed ELISA assays on HEK cells expressing β2-AR polymorphisms in order to determine β2-AR cell surface basal expression and internalization after epinephrine stimulation. We observed no differences with the WT on receptor cell surface expression and internalization for the β2-AR polymorphisms Q27E and T164I while polymorphism R16G showed an increased receptor cell surface expression and a decreased internalization post epinephrine stimulation.

More information

Item ID: 64888
DC Identifier: http://oa.upm.es/64888/
OAI Identifier: oai:oa.upm.es:64888
Deposited by: María Imbert Griñó
Deposited on: 22 Oct 2020 05:13
Last Modified: 22 Oct 2020 11:27
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