Efecto antibacteriano de nanopartículas PolymP-n Active sobre la formación de biopelículas de bacterias orales en discos de titanio

Lázaro Jiménez, Lidia (2020). Efecto antibacteriano de nanopartículas PolymP-n Active sobre la formación de biopelículas de bacterias orales en discos de titanio. Proyecto Fin de Carrera / Trabajo Fin de Grado, E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM), Madrid.

Description

Title: Efecto antibacteriano de nanopartículas PolymP-n Active sobre la formación de biopelículas de bacterias orales en discos de titanio
Author/s:
  • Lázaro Jiménez, Lidia
Contributor/s:
  • Sanz Alonso, Mariano
  • Benito Casado, Begoña
Item Type: Final Project
Degree: Grado en Biotecnología
Date: July 2020
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The oral cavity is considered the second region of the human body behind the colon, which contains the most complex microbiota. The bacteria that make up the oral microbiota, tend to form biofilms around periodontal tissue to survive. The formation of biofilms in the oral cavity can suffer an imbalance of the microbiota due to different factors, which can lead to a chronic inflammatory disease, called periodontitis, which leads to the loss of teeth due to the destruction of the oral tissues around them. Once this occurs, the use of a dental implant, usually made of titanium, is required to replace the lost teeth. The problem resides when the continuous inflammation ends up causing the destruction of the tissues with the loss of the implant, developing the so-called peri-implantitis. The antimicrobial effect of titanium disc coated by two types of nanoparticles: PolymP-n Active and Active doped with doxycycline, was examined, against an in vitro biofilm composed of Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans, representing bacteria commonly found in the mouth, during different incubation times (12, 24, 48 and 72 hours). Quantitative real-time PCR analysis was performed to assess bacterial load. It is shown that Active nanoparticles doped with doxycycline, produce a significant reduction in the number of bacteria in all species that make up the biofilm, except Porphyromonas gingivalis, being the latest to form the biofilm, where doxycicline does apparently does not reach. On the other hand, Active nanoparticles have not shown any significant antibacterial effect.

More information

Item ID: 65974
DC Identifier: http://oa.upm.es/65974/
OAI Identifier: oai:oa.upm.es:65974
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 02 Feb 2021 10:52
Last Modified: 02 Feb 2021 10:52
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