Respuesta del macrófago a superficies de CoCr funcionalizadas: estudio de la biocompatibilidad y la respuesta inflamatoria

Rubio Álamo, María (2020). Respuesta del macrófago a superficies de CoCr funcionalizadas: estudio de la biocompatibilidad y la respuesta inflamatoria. Proyecto Fin de Carrera / Trabajo Fin de Grado, E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM), Madrid.

Description

Title: Respuesta del macrófago a superficies de CoCr funcionalizadas: estudio de la biocompatibilidad y la respuesta inflamatoria
Author/s:
  • Rubio Álamo, María
Contributor/s:
  • Lozano Puerto, Rosa María
  • Peinado Lozano, Ana Belén
Item Type: Final Project
Degree: Grado en Biotecnología
Date: September 2020
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Salud y Rendimiento Humano
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Culture cell methodology is a useful experimental technique that makes possible to evaluate the behavior of the biomaterial in study. As macrophages are the specialized cells involved in the primary response to a material, a J774A.1 mouse macrophages culture cell was selected. It has been here established the suitable conditions for the maintenance of the macrophages cell culture, selecting a cell density of 15.000 cells/ml for 96-hour cell cultures and a cell density of 30.000 and 40.000 cells/ml for 72-hour cell cultures. Growth curves of J774A.1 macrophages were performed to find the most suitable conditions for a 96 hours cell culture selecting a total cell density of 10.000 cells/cm2 of growth surface. This result allows to stablish, for this culture time, data comparison between assays performed on different growth areas, moving from a format plate to another as the total number of cells per growing area unit is constant. The macrophage response to metallic CoCr-based materials and their surface modifications with ErGO and HA, has been analyzed to be used as implants in situations in which a bone repair is required. These modifications are supposed to enhance the behaviour of the material against wear and corrosion that is produced in the cellular environment where the implant will be placed. Considering the results published by Escudero et al, it is expected that the LDH/WST-1 ratio will show a lower value as time exposure to CoCrErGO increases up to 96 hours of exposure, a result that would indicate a better biocompatibility with the exposure time upon ErGO surface modification of CoCr. The effect of the additional modification of CoCrErGO with HA on macrophage biocompatibility is a study underway, but it is expected that at similar concentrations of HA to the physiological ones (3-4 mg/ml), will improve the biocompatibility of CoCr due to its lubricants properties ameliorating its behavior to wear to corrosion.

More information

Item ID: 66131
DC Identifier: http://oa.upm.es/66131/
OAI Identifier: oai:oa.upm.es:66131
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 11 Feb 2021 09:22
Last Modified: 11 Feb 2021 09:22
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