Three endo-β-mannanase genes expressed in the micropylar endosperm and in the radicle influence germination of Arabidopsis thaliana seeds

Iglesias Fernandez, Raquel and Rodriguez Gacio, Maria del Carmen and Barrero Sicilia, Cristina and Carbonero Zalduegui, Pilar and Matilla, Angel J. (2011). Three endo-β-mannanase genes expressed in the micropylar endosperm and in the radicle influence germination of Arabidopsis thaliana seeds. "Planta", v. 233 (n. 1); pp. 25-36. ISSN 0032-0935. https://doi.org/10.1007/s00425-010-1257-z.

Description

Title: Three endo-β-mannanase genes expressed in the micropylar endosperm and in the radicle influence germination of Arabidopsis thaliana seeds
Author/s:
  • Iglesias Fernandez, Raquel
  • Rodriguez Gacio, Maria del Carmen
  • Barrero Sicilia, Cristina
  • Carbonero Zalduegui, Pilar
  • Matilla, Angel J.
Item Type: Article
Título de Revista/Publicación: Planta
Date: January 2011
ISSN: 0032-0935
Volume: 233
Subjects:
Freetext Keywords: Arabidopsis – Endo-β-mannanases – Endospermic seeds – Germination – In situ hybridization – Micropylar endosperm – Seed imbibition – T-DNA insertion mutants
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Mannans are hemicellulosic polysaccharides in the plant primary cell wall (CW). Mature seeds, specially their endosperm cells, have CWs rich in mannan-based polymers that confer a strong mechanical resistance for the radicle protrusion upon germination. The rupture of the seed coat and endosperm are two sequential events during the germination of Arabidopsis thaliana. Endo-β-mannanases (MAN; EC. 3.2.1.78) are hydrolytic enzymes that catalyze cleavage of β1 → 4 bonds in the mannan-polymer. In the genome of Arabidopsis, the endo-β-mannanase (MAN) family is represented by eight members. The expression of these eight MAN genes has been systematically explored in different organs of this plant and only four of them (AtMAN7, AtMAN6, AtMAN2 and AtMAN5) are expressed in the germinating seeds. Moreover, in situ hybridization analysis shows that their transcript accumulation is restricted to the micropylar endosperm and to the radicle and this expression disappears soon after radicle emergence. T-DNA insertion mutants in these genes (K.O. MAN7, K.O. MAN6, K.O. MAN5), except that corresponding to AtMAN2 (K.O. MAN2), germinate later than the wild type (Wt). K.O. MAN6 is the most affected in the germination time course with a t 50 almost double than that of the Wt. These data suggest that AtMAN7, AtMAN5 and specially AtMAN6 are important for the germination of A. thaliana seeds by facilitating the hydrolysis of the mannan-rich endosperm cell walls.

More information

Item ID: 8225
DC Identifier: http://oa.upm.es/8225/
OAI Identifier: oai:oa.upm.es:8225
DOI: 10.1007/s00425-010-1257-z
Official URL: http://www.springerlink.com/content/t0gvr523666472q1/
Deposited by: Memoria Investigacion
Deposited on: 13 Sep 2011 08:55
Last Modified: 20 Apr 2016 17:05
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