Analysis of Ascorbate Peroxidase Genes Expressed in Resistant and Susceptible Wheat Lines Infected by Heterodera avenae.

Simonetti, Ester; Alba García, Eva; Montes, Maria Jesus; Delibes Castro, Angeles y Lopez Braña, Isidoro (2010). Analysis of Ascorbate Peroxidase Genes Expressed in Resistant and Susceptible Wheat Lines Infected by Heterodera avenae.. "Plant Cell Reports", v. 29 (n. 10); pp. 1169-1178. ISSN 0721-7714. https://doi.org/10.1007/s00299-010-0903-z.

Descripción

Título: Analysis of Ascorbate Peroxidase Genes Expressed in Resistant and Susceptible Wheat Lines Infected by Heterodera avenae.
Autor/es:
  • Simonetti, Ester
  • Alba García, Eva
  • Montes, Maria Jesus
  • Delibes Castro, Angeles
  • Lopez Braña, Isidoro
Tipo de Documento: Artículo
Título de Revista/Publicación: Plant Cell Reports
Fecha: Octubre 2010
Volumen: 29
Materias:
Palabras Clave Informales: Aegilops - Cereal cyst nematode (CCN) - Host–pathogen interaction - Reactive oxygen species (ROS) - Triticum aestivum - Ascorbate peroxidase
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Changes in ascorbate peroxidase (APX) enzyme activity in response to nematode (Heterodera avenae) attack were studied in roots of three hexaploid wheat lines carrying Cre2, Cre5, or Cre7 nematode resistance genes and the susceptible Triticum aestivum cv. Anza. A spectrophotometric analysis was carried out with root extracts of infected plants 4, 7, 11, and 14 days after nematode inoculation using uninfected plant as control. APX induction in infected resistant genotypes was similar and higher than in the susceptible control. The introgression wheat/Aegilops ventricosa H-93-8 line, carrying the Cre2 gene, and its parental line H-10-15 as susceptible control were used to analyze whether this increase of activity was correlated with the induction of APX gene expression. Genes encoding cytosolic forms of APX were induced in roots of both lines in response to nematode infection. This induction took place both earlier and with greater intensity in the resistant line than in the susceptible one, and it was also higher in the root area at the site of nematode attachment.

Más información

ID de Registro: 8467
Identificador DC: http://oa.upm.es/8467/
Identificador OAI: oai:oa.upm.es:8467
Identificador DOI: 10.1007/s00299-010-0903-z
URL Oficial: http://www.springerlink.com/content/4862ggj0p3135810/
Depositado por: Memoria Investigacion
Depositado el: 19 Ago 2011 08:47
Ultima Modificación: 20 Abr 2016 17:14
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