Regression of established subcutaneous B16-F10 murine melanoma tumors after gef gene therapy via the mitochondrial apoptotic pathway

Prados, Jose and Melguizo, Consolacion and Ortiz, Raul and Boulaiz, Houria and Carrillo, Esmeralda and Segura, Ana and Rodríguez Herva, Jose Juan and Ramos, Juan Luis and Aranega, Antonia (2010). Regression of established subcutaneous B16-F10 murine melanoma tumors after gef gene therapy via the mitochondrial apoptotic pathway. "Experimental Dermatology", v. 19 (n. 4); pp. 363-371. ISSN 0906-6705. https://doi.org/10.1111/j.1600-0625.2009.00914.x.

Description

Title: Regression of established subcutaneous B16-F10 murine melanoma tumors after gef gene therapy via the mitochondrial apoptotic pathway
Author/s:
  • Prados, Jose
  • Melguizo, Consolacion
  • Ortiz, Raul
  • Boulaiz, Houria
  • Carrillo, Esmeralda
  • Segura, Ana
  • Rodríguez Herva, Jose Juan
  • Ramos, Juan Luis
  • Aranega, Antonia
Item Type: Article
Título de Revista/Publicación: Experimental Dermatology
Date: April 2010
ISSN: 0906-6705
Volume: 19
Subjects:
Freetext Keywords: apoptosis;caspase;gef gene;melanoma;mitochondria
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

ovel treatment modalities, including gene therapy, are needed for patients with advanced melanoma. We evaluated whether the gef gene, a suicide gene from Escherichia coli, had a significant cytotoxic impact on melanoma in vivo. First, we used a non-viral gene delivery approach (pcDNA3.1/gef) to study the inhibition of melanoma cells (B16-F10) proliferation in vitro. Secondly, we used direct intra-tumoral injection of pcDNA3.1/gef complexed with jetPEI to deliver gef cDNA to rapidly growing murine melanomas. We demonstrated that gef gene not only has an antiproliferative effect on B16-F10 cells in vitro, but also induces an important decrease in melanoma tumor volume (77.7% in 8 days) in vivo. Interestingly, after gef gene treatment, melanoma showed apoptosis activation associated with the mitochondrial pathway, suggesting that the induction of this death mechanism may be an effective strategy for its treatment. Our in vivo results indicate that gef gene might become a suitable therapeutic strategy for patients with advanced melanoma.

More information

Item ID: 8480
DC Identifier: http://oa.upm.es/8480/
OAI Identifier: oai:oa.upm.es:8480
DOI: 10.1111/j.1600-0625.2009.00914.x
Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0625.2009.00914.x/full
Deposited by: Memoria Investigacion
Deposited on: 18 Aug 2011 10:02
Last Modified: 20 Apr 2016 17:14
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