Physiological and transcriptomic characterization of a fliA mutant of Pseudomonas putida KT2440

Rodríguez Herva, Jose Juan and Duque, Estrella and Molina Henares, Maria and Navarro Aviles, Gloria and Van Dillewijn, Pieter and Torre, Jesus de la and Molina Henares, Antonio J. and Sanchez de la Campa, Ana and Ran, F. Ann and Segura, Ana and Shingler, Victoria and Ramos, Juan Luis (2010). Physiological and transcriptomic characterization of a fliA mutant of Pseudomonas putida KT2440. "Environmental Microbiology Reports", v. 2 (n. 3); pp. 373-380. ISSN 1758-2229. https://doi.org/10.1111/j.1758-2229.2009.00084.x.

Description

Title: Physiological and transcriptomic characterization of a fliA mutant of Pseudomonas putida KT2440
Author/s:
  • Rodríguez Herva, Jose Juan
  • Duque, Estrella
  • Molina Henares, Maria
  • Navarro Aviles, Gloria
  • Van Dillewijn, Pieter
  • Torre, Jesus de la
  • Molina Henares, Antonio J.
  • Sanchez de la Campa, Ana
  • Ran, F. Ann
  • Segura, Ana
  • Shingler, Victoria
  • Ramos, Juan Luis
Item Type: Article
Título de Revista/Publicación: Environmental Microbiology Reports
Date: June 2010
ISSN: 1758-2229
Volume: 2
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Pseudomonas putida KT2440 encodes 23 alternative sigma factors. The fliA gene, which encodes σ28, is in a cluster with other genes involved in flagella biosynthesis and chemotaxis. Reverse transcriptase-PCR revealed that this cluster is comprised of four independent transcriptional units: flhAF, fleNfliA, cheYZA and cheBmotAB. We generated a nonpolar fliA mutant by homologous recombination and tested its motility, adhesion to biotic and abiotic surfaces, and responses to various stress conditions. The mutant strain was nonmotile and exhibited decreased capacity to bind to corn seeds, although its ability to colonize the rhizosphere of plants was unaffected. The mutant was also affected in binding to abiotic surfaces and its ability to form biofilms decreased by almost threefold. In the fliA mutant background expression of 25 genes was affected: two genes were upregulated and 23 genes were downregulated. In addition to a number of motility and chemotaxis genes, the fliA gene product is also necessary for the expression of some genes potentially involved in amino acid utilization or stress responses; however, we were unable to assign specific phenotypes linked to these genes since the fliA mutant used the same range of amino acids as the parental strain, and was as tolerant as the wild type to stress imposed by heat, antibiotics, NaCl, sodium dodecyl sulfate, H2O2 and benzoate. Based on the sequence alignment of promoters recognized by FliA and genome in silico analysis, we propose that P. putidaσ28 recognizes a TCAAG-t-N12-GCCGATA consensus sequence located between −34 and −8 and that this sequence is preferentially associated with an AT-rich upstream region.

More information

Item ID: 8488
DC Identifier: http://oa.upm.es/8488/
OAI Identifier: oai:oa.upm.es:8488
DOI: 10.1111/j.1758-2229.2009.00084.x
Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1758-2229.2009.00084.x/abstract
Deposited by: Memoria Investigacion
Deposited on: 18 Aug 2011 08:29
Last Modified: 20 Apr 2016 17:15
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