Decellularization of pericardial tissue and its impact on tensile viscoelasticity and glycosaminoglycan content

Mendoza Novelo, Birzabit; Avila, Eva; Cauich Rodríguez, Juan V.; Jorge Herrero, Eduardo; Rojo Pérez, Francisco Javier; Guinea Tortuero, Gustavo V. y Mata Mata, José L. (2011). Decellularization of pericardial tissue and its impact on tensile viscoelasticity and glycosaminoglycan content. "Acta Biomaterialia", v. 7 (n. 3); pp. 1241-1248. ISSN 1742-7061. https://doi.org/10.1016/j.actbio.2010.11.017.

Descripción

Título: Decellularization of pericardial tissue and its impact on tensile viscoelasticity and glycosaminoglycan content
Autor/es:
  • Mendoza Novelo, Birzabit
  • Avila, Eva
  • Cauich Rodríguez, Juan V.
  • Jorge Herrero, Eduardo
  • Rojo Pérez, Francisco Javier
  • Guinea Tortuero, Gustavo V.
  • Mata Mata, José L.
Tipo de Documento: Artículo
Título de Revista/Publicación: Acta Biomaterialia
Fecha: Marzo 2011
Volumen: 7
Materias:
Palabras Clave Informales: Tissue decellularization; Pericardium; Glycosaminoglycans; Tensile properties; Detergents
Escuela: E.T.S.I. Caminos, Canales y Puertos (UPM)
Departamento: Ciencia de los Materiales
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Bovine pericardium is a collagenous tissue commonly used as a natural biomaterial in the fabrication of cardiovascular devices. For tissue engineering purposes, this xenogeneic biomaterial must be decellularized to remove cellular antigens. With this in mind, three decellularization protocols were compared in terms of their effectiveness to extract cellular materials, their effect on glycosaminoglycan (GAG) content and, finally, their effect on tensile biomechanical behavior. The tissue decellularization was achieved by treatment with t-octyl phenoxy polyethoxy ethanol (Triton X-100), tridecyl polyethoxy ethanol (ATE) and alkaline treatment and subsequent treatment with nucleases (DNase/RNase). The quantified residual DNA content (3.0 ± 0.4%, 4.4 ± 0.6% and 5.6 ± 0.7% for Triton X-100, ATE and alkaline treatment, respectively) and the absence of nuclear structures (hematoxylin and eosin staining) were indicators of effective cell removal. In the same way, it was found that the native tissue GAG content decreased to 61.6 ± 0.6%, 62.7 ± 1.1% and 88.6 ± 0.2% for Triton X-100, ATE and alkaline treatment, respectively. In addition, an alteration in the tissue stress relaxation characteristics was observed after alkaline treatment. We can conclude that the three decellularization agents preserved the collagen structural network, anisotropy and the tensile modulus, tensile strength and maximum strain at failure of native tissue.

Más información

ID de Registro: 8495
Identificador DC: http://oa.upm.es/8495/
Identificador OAI: oai:oa.upm.es:8495
Identificador DOI: 10.1016/j.actbio.2010.11.017
URL Oficial: http://www.sciencedirect.com/science/journal/17427061
Depositado por: Memoria Investigacion
Depositado el: 17 Ago 2011 11:11
Ultima Modificación: 24 Sep 2014 17:13
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