In Vitro and in Vivo Enhanced Generation of Human A9 Dopamine Neurons from Neural Stem Cells by Bcl-XL.

Courtois, Elise T.; Castillo, Claudia G.; Gonzalez Seiz, Emma; Ramos Gomez, Milagros; Bueno, Carlos; Liste, Isabel y Martinez Serrano, Alberto (2010). In Vitro and in Vivo Enhanced Generation of Human A9 Dopamine Neurons from Neural Stem Cells by Bcl-XL.. "Journal of Biological Chemistry", v. 285 (n. 13); pp. 9881-9897. ISSN 0021-9258.

Descripción

Título: In Vitro and in Vivo Enhanced Generation of Human A9 Dopamine Neurons from Neural Stem Cells by Bcl-XL.
Autor/es:
  • Courtois, Elise T.
  • Castillo, Claudia G.
  • Gonzalez Seiz, Emma
  • Ramos Gomez, Milagros
  • Bueno, Carlos
  • Liste, Isabel
  • Martinez Serrano, Alberto
Tipo de Documento: Artículo
Título de Revista/Publicación: Journal of Biological Chemistry
Fecha: Marzo 2010
Volumen: 285
Materias:
Escuela: E.T.S.I. Telecomunicación (UPM)
Departamento: Tecnología Fotónica [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

Texto completo

[img] PDF (Document Portable Format) - Acceso permitido solamente a usuarios en el campus de la UPM - Se necesita un visor de ficheros PDF, como GSview, Xpdf o Adobe Acrobat Reader
Descargar (1MB)

Resumen

Human Neural Stem Cells derived from the ventral mesencephalon (VM) are powerful research tools and candidates for cell therapies in Parkinson′s disease (PD). Previous studies with VM dopaminergic neuron (DAn) precursors indicated poor growth potential and unstable phenotypical properties. Using the model cell line hVM1 (a new human fetal VM stem cell line) we have found that Bcl-XL enhances the generation of DAn from VM human neural stem cells. Mechanistically, Bcl-XL not only exerts the expected antiapoptotic effect, but it also induces proneural (NGN2, NEUROD1) and DA related transcription factors, resulting in a high yield of DAn with the correct phenotype of Substantia Nigra pars compacta (SNpc). The expression of key genes directly involved in VM/SNpc dopaminergic patterning, differentiation and maturation (EN1, LMX1B, PITX3, NURR1, VMAT2, GIRK2 and DAT) is thus enhanced by Bcl-XL. These effects on neurogenesis occur in parallel to a decrease in glia generation. These in vitro Bcl-XL effects are paralleled in vivo, after transplantation in hemiparkinsonian rats, where hVM1-Bcl-XL cells survive, integrate, and differentiate into mature DAn alleviating behavioral motor asymmetry. Bcl-XL then allows for human fetal VM stem cells to stably generate mature SNpc DAn both in vitro and in vivo, and is thus proposed as a helpful factor for the development of cell therapies for neurodegenerative conditions, PD in particular.

Más información

ID de Registro: 8599
Identificador DC: http://oa.upm.es/8599/
Identificador OAI: oai:oa.upm.es:8599
URL Oficial: http://www.jbc.org/
Depositado por: Memoria Investigacion
Depositado el: 02 Dic 2011 10:48
Ultima Modificación: 22 Sep 2014 10:35
  • Open Access
  • Open Access
  • Sherpa-Romeo
    Compruebe si la revista anglosajona en la que ha publicado un artículo permite también su publicación en abierto.
  • Dulcinea
    Compruebe si la revista española en la que ha publicado un artículo permite también su publicación en abierto.
  • Recolecta
  • e-ciencia
  • Observatorio I+D+i UPM
  • OpenCourseWare UPM