Y3IP1, A nucleus-encoded Thylakoid Protein, Cooperates with the Plastid-Encoded YCf3 Protein in Photosystem I Assembly of Tobacco and Arabidopsis

Albus, Christin A. and Ruf, Stephanie and Schöttler, Mark Aurel and Lein, Wolfgang and Kehr, Julia and Bock, Ralph (2010). Y3IP1, A nucleus-encoded Thylakoid Protein, Cooperates with the Plastid-Encoded YCf3 Protein in Photosystem I Assembly of Tobacco and Arabidopsis. "Plant Cell", v. 22 (n. 8); pp. 2838-2855. ISSN 1040-4651. https://doi.org/10.1105/tpc.110.073908.

Description

Title: Y3IP1, A nucleus-encoded Thylakoid Protein, Cooperates with the Plastid-Encoded YCf3 Protein in Photosystem I Assembly of Tobacco and Arabidopsis
Author/s:
  • Albus, Christin A.
  • Ruf, Stephanie
  • Schöttler, Mark Aurel
  • Lein, Wolfgang
  • Kehr, Julia
  • Bock, Ralph
Item Type: Article
Título de Revista/Publicación: Plant Cell
Date: January 2010
ISSN: 1040-4651
Volume: 22
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The intricate assembly of photosystem I (PSI), a large multiprotein complex in the thylakoid membrane, depends on auxiliary protein factors. One of the essential assembly factors for PSI is encoded by ycf3 (hypothetical chloroplast reading frame number 3) in the chloroplast genome of algae and higher plants. To identify novel factors involved in PSI assembly, we constructed an epitope-tagged version of ycf3 from tobacco (Nicotiana tabacum) and introduced it into the tobacco chloroplast genome by genetic transformation. Immunoaffinity purification of Ycf3 complexes from the transplastomic plants identified a novel nucleus-encoded thylakoid protein, Y3IP1 (for Ycf3-interacting protein 1), that specifically interacts with the Ycf3 protein. Subsequent reverse genetics analysis of Y3IP1 function in tobacco and Arabidopsis thaliana revealed that knockdown of Y3IP1 leads to a specific deficiency in PSI but does not result in loss of Ycf3. Our data indicate that Y3IP1 represents a novel factor for PSI biogenesis that cooperates with the plastid genome-encoded Ycf3 in the assembly of stable PSI units in the thylakoid membrane.

More information

Item ID: 8808
DC Identifier: http://oa.upm.es/8808/
OAI Identifier: oai:oa.upm.es:8808
DOI: 10.1105/tpc.110.073908
Official URL: http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-3040
Deposited by: Memoria Investigacion
Deposited on: 19 Sep 2011 10:42
Last Modified: 19 Feb 2019 15:43
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