Structural and regulatory analysis of quorum sensing in Rhizobium leguminosarum

Sanchez Cañizares, Carmen and Morata Barrado, Antonio and Cantero, L. and Jorrin, Beatriz and Ruiz Argüeso, Tomas-Andres and Imperial Ródenas, Juan and Palacios Alberti, Jose Manuel (2010). Structural and regulatory analysis of quorum sensing in Rhizobium leguminosarum. In: "XIII National Meeting of the Spanish Society of Nitrogen Fixation. II Portuguese-Spanish Congress on Nitrogen Fixation", 15/06/2011 - 18/06/2011, Zaragoza, España.

Description

Title: Structural and regulatory analysis of quorum sensing in Rhizobium leguminosarum
Author/s:
  • Sanchez Cañizares, Carmen
  • Morata Barrado, Antonio
  • Cantero, L.
  • Jorrin, Beatriz
  • Ruiz Argüeso, Tomas-Andres
  • Imperial Ródenas, Juan
  • Palacios Alberti, Jose Manuel
Item Type: Presentation at Congress or Conference (Poster)
Event Title: XIII National Meeting of the Spanish Society of Nitrogen Fixation. II Portuguese-Spanish Congress on Nitrogen Fixation
Event Dates: 15/06/2011 - 18/06/2011
Event Location: Zaragoza, España
Title of Book: Biological Nitrogen Fixation and Plant-Associated Microorganisms
Date: 2010
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

In the present work, we have studied the role of quorum-sensing regulatory systems in Rhizobium-legume symbiosis. Competition assays suggest that inactivation of quorum sensing systems significantly affects the competitiveness of Rhizobium leguminosarum bv. viciae UPM791 (Rl UPM791) when compared to other strains. Structural analysis through HPLC / mass spectrometry revealed that the signals produced by Rl UPM791 correspond to: C6-HSL, C7-HSL, C8-HSL and 3OH-C14-HSL; also, small amounts of C4-HSL have been detected. We are also analyzing the complex regulation of AHL signal molecules. We have evidence indicating that an Rl UPM791 plasmid (pUPM791d) participates in a regulatory network acting on the chromosomal system cinRI. We are using concurrent strategies (sequencing of pUPM791d and random mutagenesis) to identify the mechanism responsible for the control of pUPM791d over AHL production in Rl UPM791.

More information

Item ID: 8965
DC Identifier: http://oa.upm.es/8965/
OAI Identifier: oai:oa.upm.es:8965
Deposited by: Memoria Investigacion
Deposited on: 06 Oct 2011 10:52
Last Modified: 20 Apr 2016 17:34
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