Isolation and characterization of endosymbiotic bacteria from copper contaminated soils in Chile

Duran Wendt, David Ricardo and Soto, C. and Rubio Sanz, Laura and Cabrera Ordoñez, Ezequiel and Prieto Carbajo, Rosa Isabel and Palacios Alberti, Jose Manuel and Baginsky, C. and Brito Lopez, Maria Belen (2010). Isolation and characterization of endosymbiotic bacteria from copper contaminated soils in Chile. In: "XIII National Meeting of the Spanish Society of Nitrogen Fixation. II Portuguese-Spanish Congress on Nitrogen Fixation", 15/06/2011 - 18/06/2011, Zaragoza, España.

Description

Title: Isolation and characterization of endosymbiotic bacteria from copper contaminated soils in Chile
Author/s:
  • Duran Wendt, David Ricardo
  • Soto, C.
  • Rubio Sanz, Laura
  • Cabrera Ordoñez, Ezequiel
  • Prieto Carbajo, Rosa Isabel
  • Palacios Alberti, Jose Manuel
  • Baginsky, C.
  • Brito Lopez, Maria Belen
Item Type: Presentation at Congress or Conference (Poster)
Event Title: XIII National Meeting of the Spanish Society of Nitrogen Fixation. II Portuguese-Spanish Congress on Nitrogen Fixation
Event Dates: 15/06/2011 - 18/06/2011
Event Location: Zaragoza, España
Title of Book: Biological Nitrogen Fixation and Plant-Associated Microorganisms
Date: 2010
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Legume endosymbiotic bacteria indigenous of copper (Cu)-contaminated soils from Chile have been isolated using pea (Pisum sativum), bean (Phaseolus vulgaris) and alfalfa (Medicago sativa) as trap host plants. Highly contaminated soils only produced nodules in certain legume hosts, whereas nodulation was observed in the three legume hosts when inoculated with soils containing a low Cu concentration. A collection of 362 strains was isolated, and their levels of Cu resistance were tested in media supplemented with increasing metal concentrations and in disk diffusion assays. By these two approaches, 84 strains displaying levels of Cu resistance higher than those exhibited by the corresponding reference strains were selected. The most resistant strains isolated from alfalfa and bean nodules grew normally at 3 mM and 2.5 mM CuSO4 and were obtained from two different highly contaminated soils. Strains nodulating pea plants showed similar levels of resistance to Cu (2-2.5 mM CuSO4) and were isolated from low-contaminated soils. Our data suggest a reduction of microbial diversity in agricultural Cu-contaminated soils from Chile

More information

Item ID: 9021
DC Identifier: http://oa.upm.es/9021/
OAI Identifier: oai:oa.upm.es:9021
Deposited by: Memoria Investigacion
Deposited on: 05 Oct 2011 08:12
Last Modified: 20 Apr 2016 17:36
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