Determination of soluble toxic arsenic species in alga samples by microwave-assisted extraction and high performance liquid chromatography-hydride generation-inductively coupled plasma-atomic emission spectroscopy

Garcia Salgado, Sara and Quijano Nieto, M. Angeles and Bonilla Simon, M. Milagros (2006). Determination of soluble toxic arsenic species in alga samples by microwave-assisted extraction and high performance liquid chromatography-hydride generation-inductively coupled plasma-atomic emission spectroscopy. "Journal of Chromatography A", v. 129 (n. 1); pp. 54-60. ISSN 0021-9673. https://doi.org/10.1016/j.chroma.2006.06.083.

Description

Title: Determination of soluble toxic arsenic species in alga samples by microwave-assisted extraction and high performance liquid chromatography-hydride generation-inductively coupled plasma-atomic emission spectroscopy
Author/s:
  • Garcia Salgado, Sara
  • Quijano Nieto, M. Angeles
  • Bonilla Simon, M. Milagros
Item Type: Article
Título de Revista/Publicación: Journal of Chromatography A
Date: September 2006
ISSN: 0021-9673
Volume: 129
Subjects:
Faculty: E.U.I.T. Obras Públicas (UPM)
Department: Ingeniería Civil: Hidráulica y Energética [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

A microwave-based procedure for arsenic species extraction in alga samples (Sargassum fulvellum, Chlorella vulgaris, Hizikia fusiformis and Laminaria digitata) is described. Extraction time and temperature were tested in order to evaluate the extraction efficiency of the process. Arsenic compounds were extracted in 8 ml of deionised water at 90 °C for 5 min. The process was repeated three times. Soluble arsenic compounds extracted accounted for about 78–98% of total arsenic. The results were compared with those obtained in a previous work, where the extraction process was carried out by ultrasonic focussed probe for 30 s. Speciation studies were carried out by high performance liquid chromatography–hydride generation–inductively coupled plasma-atomic emission spectrometry (HPLC-HG-ICP-AES). The chromatographic method allowed us to separate As(III), As(V), monomethylarsonic acid and dimethylarsinic acid in less than 13 min. The chromatographic analysis of the samples allowed us to identify and quantify As(V) in Hizikia sample and Sargasso material, while the four arsenic species studied were found in Chlorella sample. In the case of Laminaria sample, none of these species was identified by HPLC-HG-ICP-AES. However, in the chromatographic analysis of this alga by HPLC-ICP-AES, an unknown arsenic species was detected.

More information

Item ID: 9713
DC Identifier: http://oa.upm.es/9713/
OAI Identifier: oai:oa.upm.es:9713
DOI: 10.1016/j.chroma.2006.06.083
Official URL: http://www.sciencedirect.com/science/journal/00219673
Deposited by: Memoria Investigacion
Deposited on: 17 Nov 2011 10:19
Last Modified: 20 Apr 2016 18:01
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