eprintid: 41451 rev_number: 15 eprint_status: archive userid: 1903 dir: disk0/00/04/14/51 datestamp: 2016-07-12 15:28:23 lastmod: 2016-07-12 15:28:23 status_changed: 2016-07-12 15:28:23 type: article metadata_visibility: show creators_name: Herrera Vásquez, Ariel creators_name: Carvallo, Loreto creators_name: Blanco, Francisca creators_name: Tobar, Mariola creators_name: Villarroel Candia, Eva creators_name: Vicente Carbajosa, Jesus creators_name: Salinas, Paula creators_name: Holuigue, Loreto creators_id: jesus.vicente@upm.es title: Transcriptional Control of Glutaredoxin GRXC9 Expression by a Salicylic Acid-Dependent and NPR1-Independent Pathway in Arabidopsis rights: by-nc-nd ispublished: pub subjects: agricultura full_text_status: public abstract: Salicylic acid (SA) is a key hormone that mediates gene transcriptional reprogramming in the context of the defense response to stress. GRXC9, coding for a CC-type glutaredoxin from Arabidopsis, is an SA-responsive gene induced early and transiently by an NPR1-independent pathway. Here, we address the mechanism involved in this SA-dependent pathway, using GRXC9 as a model gene. We first established that GRXC9 expression is induced by UVB exposure through this pathway, validating its activation in a physiological stress condition. GRXC9 promoter analyses indicate that SA controls gene transcription through two activating sequence-1 (as-1)-like elements located in its proximal region. TGA2 and TGA3, but not TGA1, are constitutively bound to this promoter region. Accordingly, the transient recruitment of RNA polymerase II to the GRXC9 promoter, as well as the transient accumulation of gene transcripts detected in SA-treated WT plants, was abolished in a knockout mutant for the TGA class II factors. We conclude that constitutive binding of TGA2 is essential for controlling GRXC9 expression, while binding of TGA3 in a lesser extent contributes to this regulation. Finally, overexpression of GRXC9 indicates that the GRXC9 protein negatively controls its own gene expression, forming part of the complex bound to the as-1-containing promoter region. These findings are integrated in a model that explains how SA controls transcription of GRXC9 in the context of the defense response to stress. date_type: published date: 2015 publication: Plant Molecular Biology Reporter volume: 33 number: 3 pagerange: 624-637 id_number: 10.1007/s11105-014-0782-5 institution: Agronomos department: Biotecnologia_2014 refereed: TRUE issn: 0735-9640 official_url: http://link.springer.com/article/10.1007/s11105-014-0782-5 citation: Herrera Vásquez, Ariel and Carvallo, Loreto and Blanco, Francisca and Tobar, Mariola and Villarroel Candia, Eva and Vicente Carbajosa, Jesus and Salinas, Paula and Holuigue, Loreto (2015). Transcriptional Control of Glutaredoxin GRXC9 Expression by a Salicylic Acid-Dependent and NPR1-Independent Pathway in Arabidopsis. "Plant Molecular Biology Reporter", v. 33 (n. 3); pp. 624-637. ISSN 0735-9640. https://doi.org/10.1007/s11105-014-0782-5 . document_url: http://oa.upm.es/41451/1/INVE_MEM_2015_231325.pdf