Citation
Luengo Oroz, Miguel Ángel and Pastor Escuredo, David and Castro González, Carlos and Faure, Emmanuel and Savy, Thierry and Lombardot, Benoit and Rubio Guivernau, José Luis and Duloquin, Louise and Ledesma Carbayo, María Jesús and Bourgine, Paul and Peyrieras, Nadine and Santos Lleo, Andres de
(2012).
3D + t Morphological Processing: Applications to Embryogenesis Image Analysis.
"Ieee Transactions on Image Processing", v. 21
(n. 8);
pp. 3518-3530.
ISSN 1057-7149.
https://doi.org/10.1109/TIP.2012.2197007.
Abstract
We propose to directly process 3D + t image sequences with mathematical morphology operators, using a new classi?cation of the 3D+t structuring elements. Several methods (?ltering, tracking, segmentation) dedicated to the analysis of 3D + t datasets of zebra?sh embryogenesis are introduced and validated through a synthetic dataset. Then, we illustrate the application of these methods to the analysis of datasets of zebra?sh early development acquired with various microscopy techniques. This processing paradigm produces spatio-temporal coherent results as it bene?ts from the intrinsic redundancy of the temporal dimension, and minimizes the needs for human intervention in semi-automatic algorithms.
