A PCR-based method for discriminating between high molecular weight glutenin subunits Bx7 and Bx7* in Triticum aestivum L

Espí Plaza, Araceli and Giraldo Carbajo, Patricia and Rodriguez de Quijano Urquiaga, Marta and Carrillo Becerril, Jose Maria (2012). A PCR-based method for discriminating between high molecular weight glutenin subunits Bx7 and Bx7* in Triticum aestivum L. "Plant Breeding" (n. 131); pp. 571-573. ISSN 0179-9541. https://doi.org/10.1111/j.1439-0523.2012.01961.x.

Description

Title: A PCR-based method for discriminating between high molecular weight glutenin subunits Bx7 and Bx7* in Triticum aestivum L
Author/s:
  • Espí Plaza, Araceli
  • Giraldo Carbajo, Patricia
  • Rodriguez de Quijano Urquiaga, Marta
  • Carrillo Becerril, Jose Maria
Item Type: Article
Título de Revista/Publicación: Plant Breeding
Date: 2012
ISSN: 0179-9541
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The correct assignment of high molecular weight glutenin subunit variants is a key task in wheat breeding. However, the traditional analysis by protein electrophoresis is sometimes difficult and not very precise. This work describes a novel DNA marker for the accurate discrimination between the Glu-B1 locus subunits Bx7 and Bx7*. The analysis of one hundred and forty two bread wheat cultivars from different countries has highlighted a great number of misclassifications in the literature that could lead to wrong conclusions in studies of the relationship between glutenin composition and wheat quality.

More information

Item ID: 15436
DC Identifier: https://oa.upm.es/15436/
OAI Identifier: oai:oa.upm.es:15436
DOI: 10.1111/j.1439-0523.2012.01961.x
Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0523.2012.01961.x/abstract
Deposited by: Memoria Investigacion
Deposited on: 23 May 2013 08:33
Last Modified: 21 Apr 2016 15:29
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