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Benito Saez, Santiago ORCID: https://orcid.org/0000-0002-1674-8246, Palomero Rodriguez, Felipe
ORCID: https://orcid.org/0000-0001-7021-3384, Morata Barrado, Antonio, Calderon Fernandez, Fernando
ORCID: https://orcid.org/0000-0003-4693-8718, Palmero Llamas, Daniel
ORCID: https://orcid.org/0000-0002-1442-1274 and Suarez Lepe, Jose Antonio
ORCID: https://orcid.org/0000-0001-8131-2142
(2012).
Identifying yeats belonging to the Brettanomyces/Dekkera genera through the use of selective-differential media.
"African Journal of Microbiology Research", v. 34
(n. 6);
pp. 6348-6357.
ISSN 1996-0808.
Title: | Identifying yeats belonging to the Brettanomyces/Dekkera genera through the use of selective-differential media |
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Author/s: |
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Item Type: | Article |
Título de Revista/Publicación: | African Journal of Microbiology Research |
Date: | 2012 |
ISSN: | 1996-0808 |
Volume: | 34 |
Subjects: | |
Faculty: | E.U.I.T. Agrícolas (UPM) |
Department: | Ciencia y Tecnología Aplicadas a la Ingeniería Técnica Agrícola [hasta 2014] |
Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
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The purpose of this work was to compare and optimise different selective and differential media to aid in isolating spoilage yeasts belonging to the Brettanomyces/Dekkera genera. Growth media containing selective and differential factors were employed. These were inoculated with strains of yeast representing Spanish oenological microbiota. Lastly, some of these isolation media were successfully applied in 24 types of wine with a high ethylphenol content, all of which were from the Haro Oenological Station (La Rioja, Spain). p-coumaric acid was determined using High performance liquid chromatography-photodiode-array detection-electrospray ionization mass spectrometry (HPLC-DAD-ESI/MS); 4-ethylphenol by using Solid phase micro extraction-gas chromatography-mass spectrometry (SPME-GC-MS); and the rest of the analysis was carried out using official OIV methodology. Actidione is the most effective selective factor for isolating Brettanomyces/Dekkera yeast genera. Other secondary selective factors (selective carbon sources, sorbic acid and ethanol as a microbicide agent) may be used successfully to eliminate potential false positivities; however, they slow growth and delay the time to obtain results.
Item ID: | 15560 |
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DC Identifier: | https://oa.upm.es/15560/ |
OAI Identifier: | oai:oa.upm.es:15560 |
Deposited by: | Memoria Investigacion |
Deposited on: | 15 Jul 2013 09:20 |
Last Modified: | 21 Apr 2016 15:41 |