Citation
Rodríguez Herva, Jose Juan and Gonzalez-Melendi de Leon, Pablo and Cuartas-Lanza, Raquel and Antúnez-Lamas, María and Río-Álvarez, Isabel and Li, Ziduo and López-Torrejón, Gema and Diaz Rodriguez, Isabel and Pozo, Juan C. del and Chakravarthy, Suma and Collmer, Alan and Rodriguez Palenzuela, Pablo and Lopez Solanilla, Emilia
(2012).
A bacterial cysteine protease effector protein interferes with photosynthesis to suppress plant innate immune responses.
"Cellular Microbiology", v. 14
(n. 5);
pp. 669-681.
ISSN 1462-5814.
https://doi.org/10.1111/j.1462-5822.2012.01749.x.
Abstract
The bacterial pathogen Pseudomonas syringae pv tomato DC3000 suppresses plant innate immunity with effector proteins injected by a type III secretion system (T3SS). The cysteine protease effector HopN1, which reduces the ability of DC3000 to elicit programmed cell death in non-host tobacco, was found to also suppress the production of defence-associated reactive oxygen species (ROS) and callose when delivered by Pseudomonas fluorescens heterologously expressing a P. syringae T3SS. Purified His 6 -tagged HopN1 was used to identify tomato PsbQ, a member of the oxygen evolving complex of photosystem II (PSII), as an interacting protein. HopN1 localized to chloroplasts and both degraded PsbQ and inhibited PSII activity in chloroplast preparations, whereas a HopN1 D299A non-catalytic mutant lost these abilities. Gene silencing of NtPsbQ in tobacco compromised ROS production and programmed cell death.
